PMID- 24210890
OWN - NLM
STAT- MEDLINE
DCOM- 20140527
LR  - 20191210
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1320
DP  - 2013 Dec 13
TI  - Halogenated phenolic compound determination in plasma and serum by solid phase 
      extraction, dansylation derivatization and liquid chromatography-positive 
      electrospray ionization-tandem quadrupole mass spectrometry.
PG  - 111-7
LID - S0021-9673(13)01687-7 [pii]
LID - 10.1016/j.chroma.2013.10.068 [doi]
AB  - A robust, sensitive and accurate method was developed for the simultaneous 
      determination in plasma and serum of suite a halogenated phenolic compounds 
      (HPCs) for which several are known to persist in the environment and analytically 
      pure standards are available. Namely, 14 congeners of hydroxylated polybrominated 
      diphenyl ethers (OH-PBDEs), six congeners of hydroxylated polychlorinated 
      biphenyls (OH-PCBs), pentachlorophenol, pentabromophenol and the flame retardant 
      tetrabromobisphenol A (TBBPA). Solid phase extraction (SPE) enriched the target 
      compounds and cleaned up the samples as a result of efficient adsorption on a 
      strong anion-exchange solid phase SPE cartridge (Oasis MAX). After final clean-up 
      the HPCs were derivatized with dansyl chloride and analyzed by liquid 
      chromatography-tandem mass spectrometry with electrospray ionization in positive 
      mode (ESI(+)). Chromatographic separation was achieved on a Luna PFP(2) column 
      (2mmx100mm, 3mum particle size) with mobile phases of water and acetonitrile (both 
      containing 0.1% formic acid). The addition of the dansyl moiety to the HPCs 
      greatly improved analyte sensitivity as the electrospray ionization efficiency 
      was enhanced. Instrument limits of detection (ILODs) for LC-ESI(+)-MS/MS analysis 
      of the HPCs were in the range of 0.01-0.07ng/mL and the method limits of 
      quantification (MLOQs) were in the range of 0.02-0.15ng/g. Recovery efficiencies 
      of the suite of HPCs ranged from 64% to 118% with relative standard deviations 
      from 2% to 12% from fortified bovine serum samples. The method was successfully 
      applied for HPC determination in representative polar bear and snapping turtle 
      plasma samples.
CI  - Crown Copyright (c) 2013. Published by Elsevier B.V. All rights reserved.
FAU - Chu, Shaogang
AU  - Chu S
AD  - Ecotoxicology and Wildlife Health Division, Science and Technology Branch, 
      Environment Canada, National Wildlife Research Centre, Carleton University, 
      Ottawa, ON K1A 0H3, Canada.
FAU - Letcher, Robert J
AU  - Letcher RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20131026
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Dansyl Compounds)
RN  - 0 (Halogens)
RN  - 0 (Phenols)
SB  - IM
MH  - Chromatography, Liquid/*methods
MH  - Dansyl Compounds/*chemistry
MH  - Halogens/*blood
MH  - Limit of Detection
MH  - Phenols/*blood
MH  - Quality Control
MH  - Reproducibility of Results
MH  - Solid Phase Extraction/*methods
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Dansylation and SPE
OT  - Halogenated phenolic compounds
OT  - LC-ESI(+)-MS/MS
OT  - Plasma
EDAT- 2013/11/12 06:00
MHDA- 2014/05/28 06:00
CRDT- 2013/11/12 06:00
PHST- 2013/07/30 00:00 [received]
PHST- 2013/10/09 00:00 [revised]
PHST- 2013/10/19 00:00 [accepted]
PHST- 2013/11/12 06:00 [entrez]
PHST- 2013/11/12 06:00 [pubmed]
PHST- 2014/05/28 06:00 [medline]
AID - S0021-9673(13)01687-7 [pii]
AID - 10.1016/j.chroma.2013.10.068 [doi]
PST - ppublish
SO  - J Chromatogr A. 2013 Dec 13;1320:111-7. doi: 10.1016/j.chroma.2013.10.068. Epub 
      2013 Oct 26.