PMID- 24211232
OWN - NLM
STAT- MEDLINE
DCOM- 20141106
LR  - 20140311
IS  - 1096-0961 (Electronic)
IS  - 1079-9796 (Linking)
VI  - 52
IP  - 4
DP  - 2014 Apr
TI  - Fluorescence in situ hybridization panel for monitoring of minimal residual 
      disease in patients with double minute chromosomes.
PG  - 208-13
LID - S1079-9796(13)00237-4 [pii]
LID - 10.1016/j.bcmd.2013.10.008 [doi]
AB  - A double minute chromosome (dmin) is a small fragment of extrachromosomal DNA 
      bearing amplified genes observed in malignancies. We investigated the incidence 
      and characteristics of dmins in hematologic malignancies, and the quantitative 
      changes during the treatment follow-up. Once a dmin was observed in conventional 
      G-banding, it was characterized using fluorescence in situ hybridization (FISH) 
      with the panel of MYC, NMYC, and MLL probes. Quantitative changes of malignant 
      cells were measured using G-banding and FISH during the follow up. Dmins were 
      observed in 1.23% of patients (6/489) at the initial diagnosis including 4 with 
      MYC amplification, 1 with MLL and 1 with NMYC. All 6 had complex karyotypes and 
      showed short overall survival (7.7 months). In follow-up specimens, FISH detected 
      dmins in 11 cases out of which G-banding detected dmins in 9 cases. The number of 
      dmins detected by FISH and G-banding did not correlate well. Amplification of 
      NMYC in dmins is reported for the first time. A FISH panel composed of frequently 
      amplified oncogenes (MYC, NMYC, and MLL) in dmins is useful for characterization 
      of dmins. FISH is a sensitive method in detecting dmins and will be useful in 
      monitoring of the minimal residual disease.
CI  - (c) 2013.
FAU - Jeon, Yongbum
AU  - Jeon Y
AD  - Department of Laboratory Medicine, Seoul National University College of Medicine, 
      101 Daehak-ro, Jongno-gu, Seoul 110-744, Republic of Korea; Department of 
      Laboratory Medicine, Seoul National University Hospital, 101 Daehak-ro, 
      Jongno-gu, Seoul 110-744, Republic of Korea.
FAU - Kim, Seon Young
AU  - Kim SY
AD  - Department of Laboratory Medicine, Seoul National University College of Medicine, 
      101 Daehak-ro, Jongno-gu, Seoul 110-744, Republic of Korea; Department of 
      Laboratory Medicine, Seoul National University Hospital, 101 Daehak-ro, 
      Jongno-gu, Seoul 110-744, Republic of Korea.
FAU - Kim, Miyoung
AU  - Kim M
AD  - Department of Laboratory Medicine, Seoul National University College of Medicine, 
      101 Daehak-ro, Jongno-gu, Seoul 110-744, Republic of Korea; Department of 
      Laboratory Medicine, Seoul National University Hospital, 101 Daehak-ro, 
      Jongno-gu, Seoul 110-744, Republic of Korea.
FAU - Park, Hyun-Kyung
AU  - Park HK
AD  - Department of Laboratory Medicine, Seoul Medical Science Institute, 7-14 
      Dongbinggo-dong Yongsan-gu, Seoul 140-809, Republic of Korea.
FAU - Lee, Sang Ho
AU  - Lee SH
AD  - Department of Laboratory Medicine, Seoul National University Hospital, 101 
      Daehak-ro, Jongno-gu, Seoul 110-744, Republic of Korea.
FAU - See, Cha Ja
AU  - See CJ
AD  - Department of Laboratory Medicine, Seoul National University Hospital, 101 
      Daehak-ro, Jongno-gu, Seoul 110-744, Republic of Korea.
FAU - Kwon, Jiseok
AU  - Kwon J
AD  - Cancer Research Institute, Seoul National University College of Medicine, 101 
      Daehak-ro, Jongno-gu, Seoul 110-744, Republic of Korea.
FAU - Lee, Dong Soon
AU  - Lee DS
AD  - Department of Laboratory Medicine, Seoul National University College of Medicine, 
      101 Daehak-ro, Jongno-gu, Seoul 110-744, Republic of Korea; Department of 
      Laboratory Medicine, Seoul National University Hospital, 101 Daehak-ro, 
      Jongno-gu, Seoul 110-744, Republic of Korea; Cancer Research Institute, Seoul 
      National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul 110-744, 
      Republic of Korea. Electronic address: soonlee@plaza.snu.ac.kr.
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131107
PL  - United States
TA  - Blood Cells Mol Dis
JT  - Blood cells, molecules & diseases
JID - 9509932
RN  - 0 (Chromatin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Bone Marrow/pathology
MH  - Child
MH  - *Chromatin
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - Fatal Outcome
MH  - Female
MH  - Hematologic Neoplasms/diagnosis/*genetics/*pathology/therapy
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - Male
MH  - Middle Aged
MH  - Neoplasm, Residual/*diagnosis
MH  - Treatment Outcome
MH  - Young Adult
OTO - NOTNLM
OT  - Double minute chromosome
OT  - Fluorescence in situ hybridization
OT  - NMYC
OT  - Oncogene
EDAT- 2013/11/12 06:00
MHDA- 2014/11/07 06:00
CRDT- 2013/11/12 06:00
PHST- 2012/08/11 00:00 [received]
PHST- 2012/11/16 00:00 [revised]
PHST- 2012/11/16 00:00 [accepted]
PHST- 2013/11/12 06:00 [entrez]
PHST- 2013/11/12 06:00 [pubmed]
PHST- 2014/11/07 06:00 [medline]
AID - S1079-9796(13)00237-4 [pii]
AID - 10.1016/j.bcmd.2013.10.008 [doi]
PST - ppublish
SO  - Blood Cells Mol Dis. 2014 Apr;52(4):208-13. doi: 10.1016/j.bcmd.2013.10.008. Epub 
      2013 Nov 7.