PMID- 24211397 OWN - NLM STAT- MEDLINE DCOM- 20140908 LR - 20131230 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) VI - 151 IP - 1 DP - 2014 TI - Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats. PG - 343-51 LID - S0378-8741(13)00786-1 [pii] LID - 10.1016/j.jep.2013.10.061 [doi] AB - ETHNOPHARMACOLOGICAL RELEVANCE: Abacopteris penangiana (Hook.) Ching (AP) is traditionally used in Chinese medicine to promote blood circulation, remove blood stasis and dampness and for the treatment of edema and inflammation. In order to further support and develop the traditional use of Abacopteris penangiana as Chinese folk medicine, the aim of this study is to investigate the protective effect of the total flavanol glycosides (TFA) from AP and its acid hydrolysate (AHT) on chronic non-bacterial prostatitis (CNP) by measuring the levels of oxidative stress and inflammatory responses in rats. MATERIALS AND METHODS: First, the antioxidant and anti-inflammatory activities of AHT and TFA were investigated. Then the experimental chronic non-bacterial prostatitis was induced by carrageenan. The prostate index (PI) and prostate specific antigen (PSA) were determined. The activities of AHT and TFA on inhibiting free radicals and oxidative stress were investigated. Subsequently, the degree of chronic inflammatory cell infiltrates, acinar changes and interstitial fibrosis were evaluated by histopathological examination. In addition, the relative inflammatory factors, tumor necrosis factor-alpha (TNF-alpha), interleukin 1beta (IL-1beta), cyclooxygenase-2 (COX-2), prostaglandin E2 (PEG2), transforming growth factor-beta1 (TGF-beta1) and connective tissue growth factor (CTGF) were measured. Finally, the prostatic expression of nuclear transcription factor-kappaB (NF-kappaB) was determined by immunohistochemistry and western blot analysis. RESULTS: The whole results showed that AHT and TFA had strong antioxidant and anti-inflammatory activities. In CNP model, AHT and TFA successfully decreased PI and PSA. The activities of antioxidant enzymes in AHT or TFA group were enhanced. Additionally, a morphometric analysis of the prostate gland of AHT or TFA treated rats demonstrated a significant reduction in chronic inflammatory cell infiltrates and interstitial fibrosis compared to model group. The reduced values of TNF-alpha, IL-1beta, COX-2, PEG2, inducible nitric oxide synthase (iNOS) and nitric oxide (NO) were observed both in AHT and TFA treated groups. Moreover, the levels of TGF-beta1 and CTGF in AHT and TFA treated groups were significantly decreased along with the alleviation of the inflammatory state of the prostate gland. Besides, the prostatic expression of NF-kappaB was inhibited. CONCLUSIONS: These results suggest that AHT and TFA have anti-prostatitis properties via inhibiting oxidative stress, NF-kappaB dependent pro-inflammatory cytokines, fibrosis-related factors and antinociceptive activity. Hence, AP represents a potential herb for the treatment of prostatitis. CI - (c) 2013 Published by Elsevier Ireland Ltd. FAU - Yang, Xian AU - Yang X AD - Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation of Hubei Province, College of Pharmacy, Tongji Medical Center, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. FAU - Yuan, Liuliu AU - Yuan L AD - Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation of Hubei Province, College of Pharmacy, Tongji Medical Center, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. FAU - Chen, Jinglou AU - Chen J AD - Department of Pharmacy, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. FAU - Xiong, Chaomei AU - Xiong C AD - Department of Pharmaceutical Analysis, Tongji Medical Center of Huazhong University of Science and Technology, Wuhan 430030, China. FAU - Ruan, Jinlan AU - Ruan J AD - Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation of Hubei Province, College of Pharmacy, Tongji Medical Center, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. Electronic address: jinlan8152@163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20131107 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 RN - 0 (Glycosides) RN - 0 (Interleukin-1beta) RN - 0 (Tumor Necrosis Factor-alpha) RN - 4Y8F71G49Q (Malondialdehyde) RN - 9000-07-1 (Carrageenan) RN - EC 1.11.1.9 (Glutathione Peroxidase) RN - EC 1.15.1.1 (Superoxide Dismutase) RN - EC 3.4.21.77 (Prostate-Specific Antigen) SB - IM MH - Animals MH - Carrageenan/*toxicity MH - Edema/chemically induced MH - Ferns/*chemistry MH - Gene Expression Regulation/drug effects MH - Glutathione Peroxidase/genetics/metabolism MH - Glycosides/chemistry/*pharmacology MH - Hydrolysis MH - Interleukin-1beta/genetics/metabolism MH - Male MH - Malondialdehyde/metabolism MH - Prostate-Specific Antigen/blood/metabolism MH - Prostatitis/*chemically induced/*drug therapy MH - Rats MH - Rats, Sprague-Dawley MH - Superoxide Dismutase/genetics/metabolism MH - Tumor Necrosis Factor-alpha/genetics/metabolism OTO - NOTNLM OT - Abacopteris penangiana OT - Inflammatory responses OT - NF-kappaB pathway OT - Oxidative stress OT - Prostatitis EDAT- 2013/11/12 06:00 MHDA- 2014/09/10 06:00 CRDT- 2013/11/12 06:00 PHST- 2013/07/11 00:00 [received] PHST- 2013/10/14 00:00 [revised] PHST- 2013/10/23 00:00 [accepted] PHST- 2013/11/12 06:00 [entrez] PHST- 2013/11/12 06:00 [pubmed] PHST- 2014/09/10 06:00 [medline] AID - S0378-8741(13)00786-1 [pii] AID - 10.1016/j.jep.2013.10.061 [doi] PST - ppublish SO - J Ethnopharmacol. 2014;151(1):343-51. doi: 10.1016/j.jep.2013.10.061. Epub 2013 Nov 7.