PMID- 24211968 OWN - NLM STAT- MEDLINE DCOM- 20140623 LR - 20131111 IS - 1878-3619 (Electronic) IS - 0959-2989 (Linking) VI - 24 IP - 1 DP - 2014 TI - Mineralization and biocompatibility of Antheraea pernyi (A. pernyi) silk sericin film for potential bone tissue engineering. PG - 815-24 LID - 10.3233/BME-130873 [doi] AB - This study aimed to investigate the mineralization of Antheraea pernyi (A. pernyi) silk sericin. Mineralization of A. pernyi sericin was performed by alternative soaking in calcium and phosphate. The inhibition of precipitation of calcium carbonate and von Kossa staining on A. pernyi sericin were tested, and the corresponding results prove that A. pernyi sericin has Ca binding activity. Scanning electron microscope (SEM) observation shows that spherical crystals could be nucleated on the A. pernyi sericin film. These crystals were confirmed to be hydroxyapatite according to FT-IR and XRD spectra, indicating that A. pernyi sericin is capable of mineralization. In addition, cell adhesion and growth activity assay demonstrate that A. pernyi sericin shows excellent biocompatibility for the growth of MG-63 cells. FAU - Yang, Mingying AU - Yang M AD - Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, 310058, PRC. FAU - Mandal, Namita AU - Mandal N FAU - Shuai, Yajun AU - Shuai Y FAU - Zhou, Guanshan AU - Zhou G FAU - Min, Sijia AU - Min S FAU - Zhu, Liangjun AU - Zhu L LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Biomed Mater Eng JT - Bio-medical materials and engineering JID - 9104021 RN - 0 (Apatites) RN - 0 (Biocompatible Materials) RN - 0 (Membranes, Artificial) RN - 0 (Sericins) RN - 91D9GV0Z28 (Durapatite) RN - SY7Q814VUP (Calcium) SB - IM MH - Animals MH - Apatites/chemistry MH - Biocompatible Materials/*chemistry MH - *Bone and Bones MH - Calcium/chemistry MH - Cell Adhesion MH - Cell Line, Tumor MH - Cell Proliferation MH - Crystallography, X-Ray MH - Durapatite/chemistry MH - Humans MH - Materials Testing MH - Membranes, Artificial MH - Microscopy, Electron, Scanning MH - Moths/*chemistry MH - Sericins/*chemistry MH - Spectroscopy, Fourier Transform Infrared MH - Surface Properties MH - Tissue Engineering/*methods MH - Tissue Scaffolds/*chemistry MH - X-Ray Diffraction OTO - NOTNLM OT - Antheraea pernyi (A. pernyi) silk sericin OT - cell adhesion and growth activity assay OT - hydroxyapatite OT - mineralization EDAT- 2013/11/12 06:00 MHDA- 2014/06/24 06:00 CRDT- 2013/11/12 06:00 PHST- 2013/11/12 06:00 [entrez] PHST- 2013/11/12 06:00 [pubmed] PHST- 2014/06/24 06:00 [medline] AID - N23480L40363L23H [pii] AID - 10.3233/BME-130873 [doi] PST - ppublish SO - Biomed Mater Eng. 2014;24(1):815-24. doi: 10.3233/BME-130873.