PMID- 24211981 OWN - NLM STAT- MEDLINE DCOM- 20140623 LR - 20131111 IS - 1878-3619 (Electronic) IS - 0959-2989 (Linking) VI - 24 IP - 1 DP - 2014 TI - Investigation on the decolorizing mechanism of Pseudomonas sp. R1 on reactive red X-3B. PG - 931-7 LID - 10.3233/BME-130887 [doi] AB - A strain of Pseudomonas aeruginosa (Pseudomonas sp. R1), which can efficiently decolorize reactive red X-3B, was isolated from activated sludge in a dye plant, and the decolorizing mechanism was explored in this paper. The result shows that Pseudomonas sp. R1 has very good capability for decolorization of reactive red X-3B and the decolorization rate is increased by 9.1% after optimization of the experimental parameters, which means that 89.6% of the reactive red can be removed. Investigation on decolorization mechanism showed that the decolorizing capability of Pseudomonassp. R1 was significantly affected after plasmids in Pseudomonassp. R1 were eliminated by acridine orange (AO). Meanwhile, E. coli DH5a could gain decolorizing capability after transformed with the plasmids. Plasmid elimination and transformation tests proved that the decolorizing gene in Pseudomonas sp. R1 exists in the plasmid. FAU - Zeng, Xinping AU - Zeng X AD - School of Life Science and Technology, Tongji University, Shanghai, 200092, China. FAU - Zhang, Min AU - Zhang M FAU - Li, Weihao AU - Li W FAU - Li, Chang AU - Li C FAU - Tang, Wenwei AU - Tang W LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Biomed Mater Eng JT - Bio-medical materials and engineering JID - 9104021 RN - 0 (Azo Compounds) RN - 0 (Coloring Agents) RN - 0 (Culture Media) RN - 0 (Naphthalenes) RN - 0 (Triazines) RN - 0 (Water Pollutants, Chemical) RN - 0 (reactive red 3B) RN - F30N4O6XVV (Acridine Orange) SB - IM MH - Acridine Orange/chemistry MH - Azo Compounds/chemistry MH - Biodegradation, Environmental MH - Coloring Agents/*chemistry MH - Culture Media/chemistry MH - Escherichia coli/metabolism MH - Genetic Engineering MH - Naphthalenes/*chemistry MH - Plasmids/metabolism MH - Pseudomonas/*metabolism MH - Triazines/*chemistry MH - Water Pollutants, Chemical/analysis MH - Water Purification/methods OTO - NOTNLM OT - Dye wastewater OT - Pseudomonas OT - azo-dye OT - bacteria decolorization OT - decolorization mechanism OT - plasmid EDAT- 2013/11/12 06:00 MHDA- 2014/06/24 06:00 CRDT- 2013/11/12 06:00 PHST- 2013/11/12 06:00 [entrez] PHST- 2013/11/12 06:00 [pubmed] PHST- 2014/06/24 06:00 [medline] AID - E2V428WK82K5G846 [pii] AID - 10.3233/BME-130887 [doi] PST - ppublish SO - Biomed Mater Eng. 2014;24(1):931-7. doi: 10.3233/BME-130887.