PMID- 24212141
OWN - NLM
STAT- MEDLINE
DCOM- 20140706
LR  - 20151119
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 942-943
DP  - 2013 Dec 30
TI  - A simple sample preparation approach based on hydrophilic solid-phase extraction 
      coupled with liquid chromatography-tandem mass spectrometry for determination of 
      endogenous cytokinins.
PG  - 31-6
LID - S1570-0232(13)00562-X [pii]
LID - 10.1016/j.jchromb.2013.10.024 [doi]
AB  - Cytokinins (CKs), a vital family of phytohormones, play important roles in the 
      regulation of shoot and root development. However, the quantification of CKs in 
      plant samples is frequently affected by the complex plant matrix. In the current 
      study, we developed a simple, rapid and efficient hydrophilic interaction 
      chromatography-solid phase extraction (HILIC-SPE) method for CKs purification. 
      CKs were extracted by acetonitrile (ACN) followed by HILIC-SPE (silica as 
      sorbents) purification. The extraction solution of plant samples could be 
      directly applied to HILIC-SPE without solvent evaporation step, which simplified 
      the analysis process. Moreover, with HILIC chromatographic retention mechanism, 
      the hydrophobic co-extracted impurities were efficiently removed. Subsequently, 
      CKs were separated by RPLC, orthogonal to the HILIC pretreatment process, and 
      detected by tandem mass spectrometry. The method exhibits high specificity and 
      recovery yield (>77.0%). Good linearities were obtained for all eight CKs ranging 
      from 0.002 to 100ngmL(-1) with correlation coefficients (r) higher than 0.9927. 
      The limits of detection (LODs, signal/noise=5) for the CKs were between 1.0 and 
      12.4pgmL(-1). Reproducibility of the method was evaluated by intra-day and 
      inter-day measurements and the results showed that relative standard deviations 
      (RSDs) were less than 10.5%. Employing this method, we successfully quantified 
      six CKs in 20mg Oryza sativa leaves and the method was also successfully applied 
      to Brassica napus (flower and leaves).
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Cai, Bao-Dong
AU  - Cai BD
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
FAU - Zhu, Jiu-Xia
AU  - Zhu JX
FAU - Shi, Zhi-Guo
AU  - Shi ZG
FAU - Yuan, Bi-Feng
AU  - Yuan BF
FAU - Feng, Yu-Qi
AU  - Feng YQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131021
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Cytokinins)
SB  - IM
MH  - Brassica napus/chemistry
MH  - Chromatography, Liquid/*methods
MH  - Cytokinins/*analysis/chemistry/*isolation & purification
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Limit of Detection
MH  - Oryza/chemistry
MH  - Plant Leaves/chemistry
MH  - Reproducibility of Results
MH  - Solid Phase Extraction/*methods
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Cytokinins
OT  - Hydrophilic interaction
OT  - Mass spectrometry
OT  - Sample preparation
EDAT- 2013/11/12 06:00
MHDA- 2014/07/07 06:00
CRDT- 2013/11/12 06:00
PHST- 2013/07/20 00:00 [received]
PHST- 2013/10/11 00:00 [revised]
PHST- 2013/10/13 00:00 [accepted]
PHST- 2013/11/12 06:00 [entrez]
PHST- 2013/11/12 06:00 [pubmed]
PHST- 2014/07/07 06:00 [medline]
AID - S1570-0232(13)00562-X [pii]
AID - 10.1016/j.jchromb.2013.10.024 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Dec 30;942-943:31-6. doi: 
      10.1016/j.jchromb.2013.10.024. Epub 2013 Oct 21.