PMID- 24213607
OWN - NLM
STAT- MEDLINE
DCOM- 20140603
LR  - 20211021
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 14
IP  - 11
DP  - 2013 Nov 7
TI  - Isolation, expression, and characterization of a hydroperoxide lyase gene from 
      cucumber.
PG  - 22082-101
LID - 10.3390/ijms141122082 [doi]
AB  - A full-length cDNA coding for hydroperoxide lyase (CsHPL) was isolated from 
      cucumber fruits of No. 26 (Southern China type) and No.14-1 (Northern China 
      type), which differed significantly in fruit flavor. The deduced amino acid 
      sequences of CsHPL from both lines show the same and significant similarity to 
      known plant HPLs and contain typical conserved domains of HPLs. The recombinant 
      CsHPL was confirmed to have 9/13-HPL enzymatic activity. Gene expression levels 
      of CsHPL were measured in different organs, especially in fruits of different 
      development stages of both lines. The HPL activities of fruit were identified 
      basing on the catalytic action of crude enzyme extracts incubating with 13-HPOD 
      (13-hydroperoxy-(9Z,12E)-octadecadienoic acid) and 13-HPOD + 9-HPOD 
      (9-hydroperoxy-(10E,12Z)-octadecadienoic acid), and volatile reaction products 
      were analyzed by GC-MS (gas chromatography-mass spectrometry). CsHPL gene 
      expression in No. 26 fruit occurred earlier than that of total HPL enzyme 
      activity and 13-HPL enzyme activity, and that in No. 14-1 fruit was consistent 
      with total HPL enzyme activity and 9-HPL enzyme activity. 13-HPL enzyme 
      activities decreased significantly and the 9-HPL enzyme activities increased 
      significantly with fruit ripening in both lines, which accounted for the higher 
      content of C6 aldehydes at 0-6 day post-anthesis (dpa) and higher content of C9 
      aldehydes at 9-12 dpa.
FAU - Wan, Xu-Hua
AU  - Wan XH
AD  - Key Laboratory of Horticultural Plant Germplasm Resources Utilization in 
      Northwest China, College of Horticulture, Northwest A&F University, Yangling 
      712100, China. wanxh@nwsuaf.edu.cn.
FAU - Chen, Shu-Xia
AU  - Chen SX
FAU - Wang, Cong-Ying
AU  - Wang CY
FAU - Zhang, Ran-Ran
AU  - Zhang RR
FAU - Cheng, Si-Qiong
AU  - Cheng SQ
FAU - Meng, Huan-Wen
AU  - Meng HW
FAU - Shen, Xiao-Qing
AU  - Shen XQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131107
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (9-hydroperoxy-10,12-octadecadienoic acid)
RN  - 0 (DNA, Complementary)
RN  - 0 (Linoleic Acids)
RN  - 0 (Lipid Peroxides)
RN  - 23017-93-8 (13-hydroperoxy-9,11-octadecadienoic acid)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 4.1.2.- (Aldehyde-Lyases)
RN  - EC 4.1.2.- (hydroperoxide lyase)
SB  - IM
MH  - Aldehyde-Lyases/biosynthesis/*chemistry/genetics/*isolation & purification
MH  - Amino Acid Sequence
MH  - *Cloning, Molecular
MH  - Cucumis sativus/*enzymology
MH  - Cytochrome P-450 Enzyme System/biosynthesis/*chemistry/genetics/*isolation & 
      purification
MH  - DNA, Complementary/genetics
MH  - Gene Expression Regulation, Plant
MH  - Hydrogen Peroxide/chemistry/metabolism
MH  - Linoleic Acids/chemistry
MH  - Lipid Peroxides/chemistry
PMC - PMC3856053
EDAT- 2013/11/12 06:00
MHDA- 2014/06/04 06:00
PMCR- 2013/11/01
CRDT- 2013/11/12 06:00
PHST- 2013/07/22 00:00 [received]
PHST- 2013/10/11 00:00 [revised]
PHST- 2013/10/14 00:00 [accepted]
PHST- 2013/11/12 06:00 [entrez]
PHST- 2013/11/12 06:00 [pubmed]
PHST- 2014/06/04 06:00 [medline]
PHST- 2013/11/01 00:00 [pmc-release]
AID - ijms141122082 [pii]
AID - ijms-14-22082 [pii]
AID - 10.3390/ijms141122082 [doi]
PST - epublish
SO  - Int J Mol Sci. 2013 Nov 7;14(11):22082-101. doi: 10.3390/ijms141122082.