PMID- 24216708
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131112
LR  - 20211021
IS  - 2072-6694 (Print)
IS  - 2072-6694 (Electronic)
IS  - 2072-6694 (Linking)
VI  - 5
IP  - 1
DP  - 2013 Mar 11
TI  - A Novel Three-Colour Fluorescence in Situ Hybridization Approach for the 
      Detection of t(7;12)(q36;p13) in Acute Myeloid Leukaemia Reveals New Cryptic 
      Three Way Translocation t(7;12;16).
PG  - 281-95
LID - 10.3390/cancers5010281 [doi]
AB  - The t(7;12)(q36;p13) translocation is a recurrent chromosome abnormality that 
      involves the ETV6 gene on chromosome 12 and has been identified in 20-30% of 
      infant patients with acute myeloid leukaemia (AML). The detection of t(7;12) 
      rearrangements relies on the use of fluorescence in situ hybridization (FISH) 
      because this translocation is hardly visible by chromosome banding methods. 
      Furthermore, a fusion transcript HLXB9-ETV6 is found in approximately 50% of 
      t(7;12) cases, making the reverse transcription PCR approach not an ideal 
      screening method. Considering the report of few cases of variant translocations 
      harbouring a cryptic t(7;12) rearrangement, we believe that the actual incidence 
      of this abnormality is higher than reported to date. The clinical outcome of 
      t(7;12) patients is believed to be poor, therefore an early and accurate 
      diagnosis is important in the clinical management and treatment. In this study, 
      we have designed and tested a novel three-colour FISH approach that enabled us 
      not only to confirm the presence of the t(7;12) in a number of patients studied 
      previously, but also to identify a cryptic t(7;12) as part of a complex 
      rearrangement. This new approach has proven to be an efficient and reliable 
      method to be used in the diagnostic setting.
FAU - Naiel, Abdulbasit
AU  - Naiel A
AD  - Leukaemia and Chromosome Research Laboratory, Division of Biosciences, Brunel 
      University, London, Middlesex UB8 3PH, UK. sabrina.tosi@brunel.ac.uk.
FAU - Vetter, Michael
AU  - Vetter M
FAU - Plekhanova, Olga
AU  - Plekhanova O
FAU - Fleischman, Elena
AU  - Fleischman E
FAU - Sokova, Olga
AU  - Sokova O
FAU - Tsaur, Grigory
AU  - Tsaur G
FAU - Harbott, Jochen
AU  - Harbott J
FAU - Tosi, Sabrina
AU  - Tosi S
LA  - eng
PT  - Journal Article
DEP - 20130311
PL  - Switzerland
TA  - Cancers (Basel)
JT  - Cancers
JID - 101526829
PMC - PMC3730311
EDAT- 2013/11/13 06:00
MHDA- 2013/11/13 06:01
PMCR- 2013/03/11
CRDT- 2013/11/13 06:00
PHST- 2013/01/31 00:00 [received]
PHST- 2013/02/28 00:00 [revised]
PHST- 2013/03/04 00:00 [accepted]
PHST- 2013/11/13 06:00 [entrez]
PHST- 2013/11/13 06:00 [pubmed]
PHST- 2013/11/13 06:01 [medline]
PHST- 2013/03/11 00:00 [pmc-release]
AID - cancers5010281 [pii]
AID - cancers-05-00281 [pii]
AID - 10.3390/cancers5010281 [doi]
PST - epublish
SO  - Cancers (Basel). 2013 Mar 11;5(1):281-95. doi: 10.3390/cancers5010281.