PMID- 24218938
OWN - NLM
STAT- MEDLINE
DCOM- 20140804
LR  - 20131113
IS  - 1009-3591 (Print)
IS  - 1009-3591 (Linking)
VI  - 19
IP  - 10
DP  - 2013 Oct
TI  - [Protective effect of pAdxsi-ERbeta adenovirus transfection on penile vascular 
      endothelium in ERbetaKO mice].
PG  - 873-9
AB  - OBJECTIVE: To investigate the effect of the overexpression of the ERbeta gene on 
      the penile vascular endothelium of ERbeta knockout (ERbetaKO) mice and its 
      molecular mechanisms. METHODS: We randomly divided 12 ERbetaKO male mice into 
      groups A (ERbetaKO + TNFalpha + pAdxsi-ERbeta) and B (ERbetaKO + TNFalpha + empty 
      virus), the former treated by pAdxsi-ERbeta adenovirus transfection, the latter 
      with empty virus, and meanwhile both injected intraperitoneally with TNFalpha at 
      6 microg per kg body weight per d for 14 days. Then we observed the erectile 
      function of the mice by APO, determined the changes of the endothelial markers 
      CD34 and vWF by immunohistochemical staining, and detected the expressions of the 
      relevant molecules in the eNOS-NO pathway by RT-PCR, Western blot and 
      immunohistochemistry. RESULTS: Compared with group B, group A showed a 
      significantly increased number of penile erections (0.50 +/- 0.55 vs 2.17 +/- 
      0.41, P < 0.05), shortened erectile latency ([28.83 +/- 1.33] min vs [24.00 +/- 
      1.27] min, P < 0.05), enriched CD34 and vWF markers (0.67 +/- 0.52 vs 1.50 +/- 
      0.55 and 0.50 +/- 0.55 vs 1.33 +/- 0.52, both P < 0.05), elevated expressions of 
      eNOS and Cam (RT-PCR: 1.38 +/- 0.03 vs 1.62 +/- 0.05 and 1.02 +/- 0.09 vs 1.42 
      +/- 0.05, both P < 0.05; Western blot: 1.27 +/- 0.04 vs 1.55 +/- 0.07 and 0.76 
      +/- 0.05 vs 0.95 +/- 0.08, both P < 0.05), and reduced expression of caveolin-1 
      (RT-PCR: 2.13 +/- 0.13 vs 1.72 +/- 0.08, P < 0.05; Western blot: 3.99 +/- 0.16 vs 
      3.40 +/- 0.14, P < 0.05). The results of RT-PCR were consistent with those of 
      Western blot. CONCLUSION: The ERbeta gene protects the penile vascular 
      endothelium via the eNOS-NO pathway.
FAU - Qiu, Dao-Xian
AU  - Qiu DX
AD  - Department of Urology and Andrology, Nanjing Maternity and Child Healthcare 
      Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, 
      China.
FAU - Ma, Jie-Hua
AU  - Ma JH
FAU - Pan, Lian-Jun
AU  - Pan LJ
FAU - Huang, Yu-Feng
AU  - Huang YF
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Nan Ke Xue
JT  - Zhonghua nan ke xue = National journal of andrology
JID - 101093592
RN  - 0 (Estrogen Receptor beta)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - EC 1.14.13.39 (Nos3 protein, mouse)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Endothelium, Vascular/*metabolism
MH  - Estrogen Receptor beta/*genetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Mice, Knockout
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase Type III/metabolism
MH  - Penis/blood supply/*metabolism
MH  - Transfection
EDAT- 2013/11/14 06:00
MHDA- 2014/08/05 06:00
CRDT- 2013/11/14 06:00
PHST- 2013/11/14 06:00 [entrez]
PHST- 2013/11/14 06:00 [pubmed]
PHST- 2014/08/05 06:00 [medline]
PST - ppublish
SO  - Zhonghua Nan Ke Xue. 2013 Oct;19(10):873-9.