PMID- 24219292
OWN - NLM
STAT- MEDLINE
DCOM- 20140606
LR  - 20220330
IS  - 1208-6002 (Electronic)
IS  - 0829-8211 (Linking)
VI  - 91
IP  - 6
DP  - 2013 Dec
TI  - 15(S)-HETE-induced angiogenesis in adipose tissue is mediated through activation 
      of PI3K/Akt/mTOR signaling pathway.
PG  - 498-505
LID - 10.1139/bcb-2013-0037 [doi]
AB  - Chronic low-grade inflammation underlies obesity and associated metabolic 
      dysfunctions. Lipoxygenase pathways are activated in adipose tissue during obese 
      conditions. Since adipogenesis is associated with angiogenesis, the present study 
      was designed to examine the role of 15-lipoxygenase metabolite, 
      15(S)-hydroxyeicosatetraenoic acid [15(S)-HETE] on angiogenesis in adipose 
      tissue. Results showed that 15(S)-HETE induced sprouting in fat pad 
      stromovascular tissues, induced morphological changes relevant to angiogenesis in 
      endothelial cells derived from adipose tissue, upregulated the production of 
      CD31, upregulated the gene level expression and production of vascular 
      endothelial growth factor (VEGF), indicating the pro-angiogenic effect of 
      15(S)-HETE. LY294002, an inhibitor of PI3K-Akt pathway, and rapamycin, inhibitor 
      of mammalian target of rapamycin (mTOR), significantly reversed the effect of 
      15(S)-HETE. 15(S)-HETE also induced activation of Akt and mTOR. These 
      observations suggest that 15(S)-HETE stimulates angiogenesis in adipose tissue 
      through activation of PI3K/Akt/mTOR signaling.
FAU - Soumya, Sasikumar J
AU  - Soumya SJ
AD  - a Department of Biochemistry, University of Kerala, Kariavattom, 
      Thiruvananthapuram, Kerala 695 581, India.
FAU - Binu, Sheela
AU  - Binu S
FAU - Helen, Antony
AU  - Helen A
FAU - Reddanna, Pallu
AU  - Reddanna P
FAU - Sudhakaran, Perumana R
AU  - Sudhakaran PR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131017
PL  - Canada
TA  - Biochem Cell Biol
JT  - Biochemistry and cell biology = Biochimie et biologie cellulaire
JID - 8606068
RN  - 0 (Chromones)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Hydroxyeicosatetraenoic Acids)
RN  - 0 (Morpholines)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (vascular endothelial growth factor A, rat)
RN  - 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
RN  - 73945-47-8 (15-hydroxy-5,8,11,13-eicosatetraenoic acid)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Adipose Tissue/blood supply/cytology/*drug effects/metabolism
MH  - Animals
MH  - Chromones/pharmacology
MH  - Endothelial Cells/cytology/*drug effects/metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Epididymis/blood supply/cytology/*drug effects/metabolism
MH  - Gene Expression Regulation
MH  - Hydroxyeicosatetraenoic Acids/metabolism/*pharmacology
MH  - Male
MH  - Morpholines/pharmacology
MH  - Neovascularization, Pathologic
MH  - Phosphatidylinositol 3-Kinases/*genetics/metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Platelet Endothelial Cell Adhesion Molecule-1/genetics/metabolism
MH  - Primary Cell Culture
MH  - Proto-Oncogene Proteins c-akt/*genetics/metabolism
MH  - Rats
MH  - Signal Transduction/drug effects
MH  - TOR Serine-Threonine Kinases/*genetics/metabolism
MH  - Tissue Culture Techniques
MH  - Vascular Endothelial Growth Factor A/genetics/metabolism
EDAT- 2013/11/14 06:00
MHDA- 2014/06/07 06:00
CRDT- 2013/11/14 06:00
PHST- 2013/11/14 06:00 [entrez]
PHST- 2013/11/14 06:00 [pubmed]
PHST- 2014/06/07 06:00 [medline]
AID - 10.1139/bcb-2013-0037 [doi]
PST - ppublish
SO  - Biochem Cell Biol. 2013 Dec;91(6):498-505. doi: 10.1139/bcb-2013-0037. Epub 2013 
      Oct 17.