PMID- 24222114
OWN - NLM
STAT- MEDLINE
DCOM- 20140107
LR  - 20220317
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 33
IP  - 11
DP  - 2013 Nov
TI  - MCP-1 promotes invasion and adhesion of human ovarian cancer cells.
PG  - 4785-90
AB  - BACKGROUND: Monocyte chemoattractant protein-1 (MCP-1) can accelerate tumor 
      progression by attracting tumor-associated macrophages. We studied the effects of 
      MCP-1 on SKOV-3 cells in order to investigate MCP-1 biological activity ovarian 
      cancer. MATERIALS AND METHODS: A SKOV-3 cell invasion assay (Transwell assay) and 
      cell adhesion assay (96-well assay) were performed. Immunohistochemical staining 
      for C-C motif chemokine receptor-2 (CCR2), a receptor for MCP-1, was also 
      performed on cultured SKOV-3 cells. RESULTS: Migration and adhesion of 
      MCP-1-treated SKOV-3 cells were significantly increased compared to untreated 
      cells (p<0.01). A CCR2 antagonist attenuated the invasion and adhesion of 
      MCP-1-treated cells. CCR2 was expressed in the cytoplasm of SKOV-3 cells. 
      CONCLUSION: MCP-1 promoted invasion and adhesion of ovarian cancer cells, and a 
      CCR2 antagonist attenuated the effects of MCP-1 in vitro. These data suggest that 
      MCP-1 is a potential therapeutic target for ovarian cancer therapy.
FAU - Furukawa, Shigenori
AU  - Furukawa S
AD  - Department of Obstetrics and Gynecology, Fukushima Medical University School of 
      Medicine, 1-Hikarigaoka, Fukushima City, 960-1295, Japan. s-furu@fmu.ac.jp.
FAU - Soeda, Shu
AU  - Soeda S
FAU - Kiko, Yuichiro
AU  - Kiko Y
FAU - Suzuki, Osamu
AU  - Suzuki O
FAU - Hashimoto, Yuko
AU  - Hashimoto Y
FAU - Watanabe, Takafumi
AU  - Watanabe T
FAU - Nishiyama, Hiroshi
AU  - Nishiyama H
FAU - Tasaki, Kazuhiro
AU  - Tasaki K
FAU - Hojo, Hiroshi
AU  - Hojo H
FAU - Abe, Masafumi
AU  - Abe M
FAU - Fujimori, Keiya
AU  - Fujimori K
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Chemokine CCL2)
RN  - 0 (Receptors, CCR2)
SB  - IM
MH  - *Cell Adhesion
MH  - *Cell Movement
MH  - Chemokine CCL2/*metabolism
MH  - Cystadenocarcinoma, Serous/metabolism/*pathology
MH  - Female
MH  - Humans
MH  - Neoplasm Invasiveness
MH  - Ovarian Neoplasms/metabolism/*pathology
MH  - Receptors, CCR2/*antagonists & inhibitors/metabolism
MH  - Tumor Cells, Cultured
OTO - NOTNLM
OT  - C-C motif chemokine receptor-2 (CCR2) antagonist
OT  - Ovarian cancer
OT  - SKOV-3 cell line
OT  - monocyte chemoattractant protein-1 (MCP-1)
OT  - tumor-associated macrophages (TAMs)
EDAT- 2013/11/14 06:00
MHDA- 2014/01/08 06:00
CRDT- 2013/11/14 06:00
PHST- 2013/11/14 06:00 [entrez]
PHST- 2013/11/14 06:00 [pubmed]
PHST- 2014/01/08 06:00 [medline]
AID - 33/11/4785 [pii]
PST - ppublish
SO  - Anticancer Res. 2013 Nov;33(11):4785-90.