PMID- 24223654
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220316
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 6
IP  - 5
DP  - 2013 Nov
TI  - Nerve growth factor exacerbates allergic lung inflammation and airway remodeling 
      in a rat model of chronic asthma.
PG  - 1251-1258
AB  - Nerve growth factor (NGF) is critical in the pathogenesis of allergic airway 
      inflammation in vivo and induces proliferation of airway smooth muscle cells and 
      matrix metalloproteinase-9 (MMP-9) expression in vitro. However, the effects of 
      NGF on chronic pulmonary diseases of allergic origin remain unknown. To 
      investigate the effects of NGF on lung inflammation and airway remodeling, 32 
      Wistar rats were randomly divided into four groups: control, NGF, ovalbumin (OVA) 
      and anti-rat-beta-NGF antibody (anti-NGF). Aerosolized OVA was administered to the 
      rats in the NGF, OVA and anti-NGF groups to generate the asthmatic rat model, and 
      NGF or anti-NGF was administered 3 h prior to OVA inhalation every two days. On 
      day 70, bronchial responsiveness tests, bronchoalveolar lavage (BAL) and cell 
      counting were conducted. The levels of serum OVA-specific immunoglobulin E (IgE) 
      and of T-helper cell type-2 (Th2) cytokines [interleukin (IL)-4 and IL-13] in the 
      BAL fluid were measured by enzyme-linked immunosorbent assay. The expression 
      levels of NGF protein and MMP-9 mRNA, and the activity of MMP-9 in the lungs were 
      detected by western blot analysis, quantitative polymerase chain reaction and 
      gelatin zymography analysis, respectively. Our results showed that NGF 
      significantly increased eosinophilic airway inflammation, persistent airway 
      hyperresponsiveness (AHR), the serum levels of OVA-specific IgE and the levels of 
      Th2 cytokines in the BAL fluid, and also increased the expression levels and 
      activity of MMP-9. However, anti-NGF treatment significantly inhibited 
      eosinophilic airway inflammation, persistent AHR and airway remodeling. The 
      results showed that NGF may have exacerbated the development of airway 
      inflammation, AHR and airway remodeling through a Th2 pathway and by increasing 
      the level of MMP-9 expression. Therefore, anti-NGF is potentially beneficial for 
      preventing and treating patients with asthma.
FAU - Yang, Yun-Gang
AU  - Yang YG
AD  - Department of Pediatric Pulmonology, Shengjing Hospital of China Medical 
      University, Shenyang, Liaoning 100004, P.R. China.
FAU - Tian, Wei-Min
AU  - Tian WM
FAU - Zhang, Han
AU  - Zhang H
FAU - Li, Miao
AU  - Li M
FAU - Shang, Yun-Xiao
AU  - Shang YX
LA  - eng
PT  - Journal Article
DEP - 20130902
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC3820700
OTO - NOTNLM
OT  - T-helper cell type-2 cytokines
OT  - airway remodeling
OT  - asthma
OT  - matrix metalloproteinase-9
OT  - nerve growth factor
EDAT- 2013/11/14 06:00
MHDA- 2013/11/14 06:01
PMCR- 2013/09/02
CRDT- 2013/11/14 06:00
PHST- 2013/04/20 00:00 [received]
PHST- 2013/08/20 00:00 [accepted]
PHST- 2013/11/14 06:00 [entrez]
PHST- 2013/11/14 06:00 [pubmed]
PHST- 2013/11/14 06:01 [medline]
PHST- 2013/09/02 00:00 [pmc-release]
AID - etm-06-05-1251 [pii]
AID - 10.3892/etm.2013.1284 [doi]
PST - ppublish
SO  - Exp Ther Med. 2013 Nov;6(5):1251-1258. doi: 10.3892/etm.2013.1284. Epub 2013 Sep 
      2.