PMID- 24223964
OWN - NLM
STAT- MEDLINE
DCOM- 20140818
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 11
DP  - 2013
TI  - Plasticity of migrating CD1b+ and CD1b- lymph dendritic cells in the promotion of 
      Th1, Th2 and Th17 in response to Salmonella and helminth secretions.
PG  - e79537
LID - 10.1371/journal.pone.0079537 [doi]
LID - e79537
AB  - Dendritic cells (DCs) are pivotal in the development of specific T-cell responses 
      to control pathogens, as they govern both the initiation and the polarization of 
      adaptive immunity. To investigate the capacities of migrating DCs to respond to 
      pathogens, we used physiologically generated lymph DCs (L-DCs). The flexible 
      polarization of L-DCs was analysed in response to Salmonella or helminth 
      secretions known to induce different T cell responses. Mature conventional 
      CD1b(+) L-DCs showed a predisposition to promote pro-inflammatory (IL-6), pro-Th1 
      (IL-12p40) and anti-inflammatory (IL-10) responses which were amplified by 
      Salmonella, and limited to only IL-6 induction by helminth secretions. The other 
      major population of L-DCs did not express the CD1b molecule and displayed 
      phenotypic features of immaturity compared to CD1b(+) L-DCs. Salmonella infection 
      reduced the constitutive expression of TNF-alpha and IL-4 mRNA in CD1b(-) L-DCs, 
      whereas this expression was not affected by helminth secretions. The cytokine 
      response of T cells promoted by L-DCs was analysed in T cell subsets after 
      co-culture with Salmonella or helminth secretion-driven CD1b(+) or CD1b(-) L-DCs. 
      T cells preferentially expressed the IL-17 gene, and to a lesser extent the IFN-gamma 
      and IL-10 genes, in response to Salmonella-driven CD1b(+) L-DCs, whereas a 
      preferential IL-10, IFN-gamma and IL-17 gene expression was observed in response to 
      Salmonella-driven CD1b(-) L-DCs. In contrast, a predominant IL-4 and IL-13 gene 
      expression by CD4(+) and CD8(+) T cells was observed after stimulation of CD1b(+) 
      and CD1b(-) L-DCs with helminth secretions. These results show that mature 
      conventional CD1b(+) L-DCs maintain a flexible capacity to respond differently to 
      pathogens, that the predisposition of CD1b(-) L-DCs to promote a Th2 response can 
      be oriented towards other Th responses, and finally that the modulation of 
      migrating L-DCs responses is controlled more by the pathogen encountered than the 
      L-DC subsets.
FAU - Olivier, Michel
AU  - Olivier M
AD  - UR1282 Infectiologie et Sante Publique, INRA, Nouzilly, France.
FAU - Foret, Benjamin
AU  - Foret B
FAU - Le Vern, Yves
AU  - Le Vern Y
FAU - Kerboeuf, Dominique
AU  - Kerboeuf D
FAU - Guilloteau, Laurence A
AU  - Guilloteau LA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131105
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antigens, CD1)
RN  - 0 (CD1b antigen)
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Antigens, CD1/*metabolism
MH  - *Cell Movement
MH  - Cytokines/genetics/metabolism
MH  - Dendritic Cells/*cytology/*immunology/metabolism/microbiology
MH  - Female
MH  - Helminths/*physiology
MH  - Salmonella enterica/*physiology
MH  - Sheep
MH  - T-Lymphocytes, Helper-Inducer/*immunology
MH  - Th1 Cells/immunology
MH  - Th17 Cells/immunology
MH  - Th2 Cells/immunology
PMC - PMC3818231
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/11/14 06:00
MHDA- 2014/08/19 06:00
PMCR- 2013/11/05
CRDT- 2013/11/14 06:00
PHST- 2013/08/19 00:00 [received]
PHST- 2013/09/30 00:00 [accepted]
PHST- 2013/11/14 06:00 [entrez]
PHST- 2013/11/14 06:00 [pubmed]
PHST- 2014/08/19 06:00 [medline]
PHST- 2013/11/05 00:00 [pmc-release]
AID - PONE-D-13-34109 [pii]
AID - 10.1371/journal.pone.0079537 [doi]
PST - epublish
SO  - PLoS One. 2013 Nov 5;8(11):e79537. doi: 10.1371/journal.pone.0079537. eCollection 
      2013.