PMID- 24225162
OWN - NLM
STAT- MEDLINE
DCOM- 20140813
LR  - 20181202
IS  - 1748-605X (Electronic)
IS  - 1748-6041 (Linking)
VI  - 8
IP  - 6
DP  - 2013 Dec
TI  - 3D scaffold of PLLA/pearl and PLLA/nacre powder for bone regeneration.
PG  - 065001
LID - 10.1088/1748-6041/8/6/065001 [doi]
AB  - Naturally occurring pearl and its derivatives have recently gained interest in 
      bone regeneration due to their bioactive characteristics and good mechanical 
      properties. In this study, three-dimensional scaffolds composed of poly-l-lactide 
      (PLLA)/aragonite pearl powder, PLLA/vaterite pearl powder and PLLA/nacre powder 
      were fabricated by freeze-drying. Scanning electron microscope (SEM) images 
      indicated that the addition of powder made no visible difference to the 
      morphology of the composite scaffolds. These composite scaffolds were found to 
      have nearly twice the compressive strength and compressive modulus of the pure 
      PLLA scaffold. X-ray diffraction patterns reveal that both PLLA/aragonite and 
      PLLA/nacre composite scaffolds have pure aragonite crystals as their inorganic 
      component, while PLLA/vaterite has pure vaterite crystals. The attachment and 
      morphology of rat bone marrow-derived mesenchymal stem cells (rBMSCs) on 
      scaffolds was observed by the SEM. The proliferation and osteogenic 
      differentiation of rBMSCs on composite scaffolds was also investigated. The 
      results indicate that PLLA/aragonite and PLLA/nacre scaffolds better stimulate 
      cell proliferation and alkaline phosphatase activity than the PLLA scaffold. 
      However, the PLLA/vaterite scaffold appears to decrease rBMSCs proliferation as 
      well as the osteogenic differentiation, possibly due to the high pH of the 
      solution containing PLLA/vaterite.
FAU - Liu, Yuansheng
AU  - Liu Y
AD  - State Key Laboratory of New Ceramics and Fine Processing, School of Materials 
      Science and Engineering, Tsinghua University, Beijing 100084, People's Republic 
      of China.
FAU - Huang, Qianli
AU  - Huang Q
FAU - Feng, Qingling
AU  - Feng Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131114
PL  - England
TA  - Biomed Mater
JT  - Biomedical materials (Bristol, England)
JID - 101285195
RN  - 0 (Biocompatible Materials)
RN  - 0 (Bone Substitutes)
RN  - 0 (Nacre)
RN  - 0 (Polyesters)
RN  - 0 (Polymers)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 459TN2L5F5 (poly(lactide))
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - Adsorption
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Biocompatible Materials/chemistry
MH  - Biomechanical Phenomena
MH  - *Bone Regeneration
MH  - Bone Substitutes/*chemistry
MH  - Calcium Carbonate/chemistry
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Compressive Strength
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Lactic Acid/chemistry
MH  - Materials Testing
MH  - Mesenchymal Stem Cells/cytology/metabolism
MH  - Microscopy, Electron, Scanning
MH  - Nacre/chemistry
MH  - Osteoblasts/cytology/metabolism
MH  - Osteogenesis
MH  - Polyesters
MH  - Polymers/chemistry
MH  - Porosity
MH  - Protein Binding
MH  - Rats
MH  - Surface Properties
MH  - *Tissue Engineering
MH  - Tissue Scaffolds/*chemistry
MH  - X-Ray Diffraction
EDAT- 2013/11/15 06:00
MHDA- 2014/08/15 06:00
CRDT- 2013/11/15 06:00
PHST- 2013/11/15 06:00 [entrez]
PHST- 2013/11/15 06:00 [pubmed]
PHST- 2014/08/15 06:00 [medline]
AID - 10.1088/1748-6041/8/6/065001 [doi]
PST - ppublish
SO  - Biomed Mater. 2013 Dec;8(6):065001. doi: 10.1088/1748-6041/8/6/065001. Epub 2013 
      Nov 14.