PMID- 24226795
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131115
LR  - 20211021
IS  - 1053-0509 (Print)
IS  - 1053-0509 (Linking)
VI  - 5
IP  - 3
DP  - 1995 Sep
TI  - Polarized fluorescence and absorption spectroscopy of 
      1,32-dihydroxy-dotriacontane-bis-rhodamine 101 ester. A new and lipid 
      bilayer-spanning probe.
PG  - 279-84
LID - 10.1007/BF00723899 [doi]
AB  - We report on the properties of 1,32-dihydroxy-dotriacontane-bis-rhodamine 101 
      ester (Rh101C32Rh101) in lipid bilayers of 
      1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and in liquid solvents. The 
      results are compared with those of rhodamine 101 octadecanyl ester (Rh101C18). 
      Both molecules are solubilized in the lipid bilayer and the Rh101 moieties are 
      anchored in the lipid-water interface, so that the electronic transition dipole 
      moments (S 0 <-->S 1) are oriented preferentially in the plane of the bilayer. At 
      low concentrations of the dyes in lipid bilayers of DOPC, the fluorescence 
      relaxation is single exponential with a lifetime of tau=4.9+/-0.2 ns. The relative 
      fluorescence quantum yield of PhiC32/PhiC18  approximately  0.95 in DOPC vesicles. These results 
      strongly suggest that only a small fraction of the Rh101C32Rh101 molecules are 
      quenched, by, for example, intra- or intermolecular dimers in the ground state at 
      mole fractions of less than 0.1% in the lipid bilayers. For Rh101C32Rh101 in 
      lipid vesicles, the steady-state and time-resolved fluorescence anisotropies are 
      compatible with efficient intramolecular electronic energy transfer. It is 
      concluded that nearly every Rh101C32Rh101 molecule is spanning across the lipid 
      bilayer of DOPC.
FAU - Karolin, J
AU  - Karolin J
AD  - Department of Physical Chemistry, University of Umea, S-901 87, Umea, Sweden.
FAU - Bogen, S T
AU  - Bogen ST
FAU - Johansson, L B
AU  - Johansson LB
FAU - Molotkovsky, J G
AU  - Molotkovsky JG
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Fluoresc
JT  - Journal of fluorescence
JID - 9201341
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
CRDT- 2013/11/15 06:00
PHST- 1994/07/04 00:00 [received]
PHST- 1994/10/20 00:00 [accepted]
PHST- 1994/10/10 00:00 [revised]
PHST- 2013/11/15 06:00 [entrez]
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
AID - 10.1007/BF00723899 [doi]
PST - ppublish
SO  - J Fluoresc. 1995 Sep;5(3):279-84. doi: 10.1007/BF00723899.