PMID- 24227632
OWN - NLM
STAT- MEDLINE
DCOM- 20140317
LR  - 20161125
IS  - 1535-3699 (Electronic)
IS  - 1535-3699 (Linking)
VI  - 239
IP  - 1
DP  - 2014 Jan
TI  - Magnetic resonance imaging tracking of ultra small superparamagnetic iron oxide 
      labeled rabbit dendritic cells.
PG  - 13-23
LID - 10.1177/1535370213508712 [doi]
AB  - Dendritic cells (DCs) play an important role in atherosclerosis plaque formation, 
      but the mechanism has not been elucidated clearly. This study is designed to 
      establish a method for tracing DCs in vivo facilitating the investigation of the 
      DCs' specific roles in atherosclerosis. Rabbit DCs labeled by different 
      concentrations of ultra small superparamagnetic iron oxide (USPIO) were injected 
      into atherosclerosis rabbit model and traced with magnetic resonance imaging 
      (MRI). Results showed that USPIO labeling nearly have no cytotoxicity to DCs in 
      low concentrations (<500 microg/mL) but induced some decrease of cell viability at 
      high concentrations (>500 microg/mL). Moreover, USPIO labeling, from 200 to 
      2000 microg/mL, caused a dose-dependent decrease of the mitochondrial membrane 
      potential in DCs. The high labeling concentration (2000 microg/mL) triggered necrosis 
      instead of apoptosis in DCs. By T2WI and fs T2WI sequence imaging comparison, DCs 
      were found to exist in rabbit abdominal artery plaques after 24 h of 
      transplantation and in spleen after one week detected by Prussian blue staining 
      of tissue sections. We concluded that about 200 microg/mL USPIO is ideal to 
      effectively label DCs for MRI tracing in vivo without a threat to cell viability. 
      Combining USPIO labeling and MRI to track the movement of injected DCs in vivo is 
      a feasible method.
FAU - Zhou, Jing
AU  - Zhou J
AD  - College of Biophotonics, South China Normal University, Guangzhou 510631, China.
FAU - Yang, Fuyuan
AU  - Yang F
FAU - Zhou, Quan
AU  - Zhou Q
FAU - Yang, Kanrong
AU  - Yang K
FAU - Chen, Wenli
AU  - Chen W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131113
PL  - Switzerland
TA  - Exp Biol Med (Maywood)
JT  - Experimental biology and medicine (Maywood, N.J.)
JID - 100973463
RN  - 0 (Ferric Compounds)
RN  - 0 (Magnetite Nanoparticles)
RN  - 1K09F3G675 (ferric oxide)
SB  - IM
MH  - Animals
MH  - Cell Survival/drug effects
MH  - Dendritic Cells/*diagnostic imaging/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Ferric Compounds/adverse effects/chemistry/*pharmacology
MH  - *Magnetic Resonance Imaging
MH  - *Magnetite Nanoparticles
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Necrosis/chemically induced/diagnostic imaging/metabolism
MH  - Rabbits
MH  - Radiography
MH  - Spleen/diagnostic imaging
OTO - NOTNLM
OT  - Ultra small superparamagnetic iron oxide
OT  - atherosclerotic rabbit model
OT  - dendritic cells
OT  - magnetic resonance imaging
EDAT- 2013/11/15 06:00
MHDA- 2014/03/19 06:00
CRDT- 2013/11/15 06:00
PHST- 2013/11/15 06:00 [entrez]
PHST- 2013/11/15 06:00 [pubmed]
PHST- 2014/03/19 06:00 [medline]
AID - 1535370213508712 [pii]
AID - 10.1177/1535370213508712 [doi]
PST - ppublish
SO  - Exp Biol Med (Maywood). 2014 Jan;239(1):13-23. doi: 10.1177/1535370213508712. 
      Epub 2013 Nov 13.