PMID- 24227775
OWN - NLM
STAT- MEDLINE
DCOM- 20140218
LR  - 20211021
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 191
IP  - 12
DP  - 2013 Dec 15
TI  - Batf3 and Id2 have a synergistic effect on Irf8-directed classical CD8alpha+ 
      dendritic cell development.
PG  - 5993-6001
LID - 10.4049/jimmunol.1203541 [doi]
AB  - Dendritic cells (DCs) are heterogeneous cell populations represented by different 
      subtypes, each varying in terms of gene expression patterns and specific 
      functions. Recent studies identified transcription factors essential for the 
      development of different DC subtypes, yet molecular mechanisms for the 
      developmental program and functions remain poorly understood. In this study, we 
      developed and characterized a mouse DC progenitor-like cell line, designated DC9, 
      from Irf8(-/-) bone marrow cells as a model for DC development and function. 
      Expression of Irf8 in DC9 cells led to plasmacytoid DCs and CD8alpha(+) DC-like 
      cells, with a concomitant increase in plasmacytoid DC- and CD8alpha(+) DC-specific 
      gene transcripts and induction of type I IFNs and IL12p40 following TLR ligand 
      stimulation. Irf8 expression in DC9 cells led to an increase in Id2 and Batf3 
      transcript levels, transcription factors shown to be important for the 
      development of CD8alpha(+) DCs. We show that, without Irf8, expression of Id2 and 
      Batf3 was not sufficient for directing classical CD8alpha(+) DC development. When 
      coexpressed with Irf8, Batf3 and Id2 had a synergistic effect on classical 
      CD8alpha(+) DC development. We demonstrate that Irf8 is upstream of Batf3 and Id2 in 
      the classical CD8alpha(+) DC developmental program and define the hierarchical 
      relationship of transcription factors important for classical CD8alpha(+) DC 
      development.
FAU - Jaiswal, Hemant
AU  - Jaiswal H
AD  - Laboratory of Innate Immunity, National Institute of Immunology, New Delhi 
      110067, India;
FAU - Kaushik, Monika
AU  - Kaushik M
FAU - Sougrat, Rachid
AU  - Sougrat R
FAU - Gupta, Monica
AU  - Gupta M
FAU - Dey, Anup
AU  - Dey A
FAU - Verma, Rohit
AU  - Verma R
FAU - Ozato, Keiko
AU  - Ozato K
FAU - Tailor, Prafullakumar
AU  - Tailor P
LA  - eng
GR  - Z99 HD999999/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131113
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Basic-Leucine Zipper Transcription Factors)
RN  - 0 (CD8 Antigens)
RN  - 0 (CD8 antigen, alpha chain)
RN  - 0 (Idb2 protein, mouse)
RN  - 0 (Inhibitor of Differentiation Protein 2)
RN  - 0 (Interferon Regulatory Factors)
RN  - 0 (Interferon-alpha)
RN  - 0 (Interleukin-12 Subunit p40)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (SNFT protein, mouse)
RN  - 0 (flt3 ligand protein)
RN  - 0 (interferon regulatory factor-8)
SB  - IM
MH  - Animals
MH  - Basic-Leucine Zipper Transcription Factors/biosynthesis/genetics/*physiology
MH  - CD8 Antigens/analysis
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - Dendrites/ultrastructure
MH  - Dendritic Cells/chemistry/classification/*cytology/ultrastructure
MH  - Gene Expression Regulation/*immunology
MH  - Hematopoietic Stem Cells/cytology
MH  - Inhibitor of Differentiation Protein 2/biosynthesis/genetics/*physiology
MH  - Interferon Regulatory Factors/biosynthesis/genetics/*physiology
MH  - Interferon-alpha/biosynthesis/genetics
MH  - Interleukin-12 Subunit p40/biosynthesis/genetics
MH  - Membrane Proteins/pharmacology
MH  - Mice
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Recombinant Fusion Proteins/metabolism
MH  - Repressor Proteins/biosynthesis/genetics/*physiology
MH  - Transduction, Genetic
PMC - PMC4805111
MID - NIHMS768648
EDAT- 2013/11/15 06:00
MHDA- 2014/02/19 06:00
PMCR- 2016/03/23
CRDT- 2013/11/15 06:00
PHST- 2013/11/15 06:00 [entrez]
PHST- 2013/11/15 06:00 [pubmed]
PHST- 2014/02/19 06:00 [medline]
PHST- 2016/03/23 00:00 [pmc-release]
AID - jimmunol.1203541 [pii]
AID - 10.4049/jimmunol.1203541 [doi]
PST - ppublish
SO  - J Immunol. 2013 Dec 15;191(12):5993-6001. doi: 10.4049/jimmunol.1203541. Epub 
      2013 Nov 13.