PMID- 24231291
OWN - NLM
STAT- MEDLINE
DCOM- 20140917
LR  - 20140203
IS  - 1618-0984 (Electronic)
IS  - 0723-2020 (Linking)
VI  - 37
IP  - 1
DP  - 2014 Feb
TI  - "Candidatus Sonnebornia yantaiensis", a member of candidate division OD1, as 
      intracellular bacteria of the ciliated protist Paramecium bursaria (Ciliophora, 
      Oligohymenophorea).
PG  - 35-41
LID - S0723-2020(13)00157-4 [pii]
LID - 10.1016/j.syapm.2013.08.007 [doi]
AB  - An intracellular bacterium was discovered in an isolate of Paramecium bursaria 
      from a freshwater pond in Yantai, China. The bacteria were abundant and 
      exclusively found in the cytoplasm of the host which, along with the green alga 
      Chlorella, formed a three-partner consortium that could survive in pure water for 
      at least one week. Cloning, sequencing and phylogenetic analysis of the bacterial 
      16S rRNA gene showed that the bacterium belonged to the uncultured candidate 
      division OD1, which usually forms part of the rare biosphere. Transmission 
      electron microscopy and fluorescence in situ hybridization (FISH) with specific 
      probes showed that the bacteria were usually located close to the perialgal 
      membranes of endosymbiotic Chlorella cells, and occasionally irregularly 
      distributed throughout the host cytoplasm. The name "Candidatus Sonnebornia 
      yantaiensis" gen. nov., sp. nov. is proposed for the new bacterium. A strongly 
      supported monophyletic subclade, OD1-p, which included the new species, was 
      recognized and this study highlights that protists can be important hosts for 
      rare bacterial taxa.
CI  - Copyright (c) 2013 Elsevier GmbH. All rights reserved.
FAU - Gong, Jun
AU  - Gong J
AD  - Microbial Ecology Group, Key Laboratory of Coastal Biology and Bioresource 
      Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of 
      Sciences, Yantai 264003, China; College of Life Sciences, South China Normal 
      University, Guangzhou 510631, China. Electronic address: jgong@yic.ac.cn.
FAU - Qing, Yao
AU  - Qing Y
AD  - Microbial Ecology Group, Key Laboratory of Coastal Biology and Bioresource 
      Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of 
      Sciences, Yantai 264003, China; College of Life Sciences, South China Normal 
      University, Guangzhou 510631, China.
FAU - Guo, Xiaohong
AU  - Guo X
AD  - Microbial Ecology Group, Key Laboratory of Coastal Biology and Bioresource 
      Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of 
      Sciences, Yantai 264003, China.
FAU - Warren, Alan
AU  - Warren A
AD  - Department of Life Sciences, Natural History Museum, London SW7 5BD, United 
      Kingdom.
LA  - eng
SI  - GENBANK/KC495060
SI  - GENBANK/KC495061
SI  - GENBANK/KC495062
SI  - GENBANK/KC495063
SI  - GENBANK/KC495064
SI  - GENBANK/KC495065
SI  - GENBANK/KC495066
SI  - GENBANK/KC495067
SI  - GENBANK/KC495068
SI  - GENBANK/KC495069
SI  - GENBANK/KC495070
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131111
PL  - Germany
TA  - Syst Appl Microbiol
JT  - Systematic and applied microbiology
JID - 8306133
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Bacteria/*classification/genetics/*isolation & purification/ultrastructure
MH  - China
MH  - Cluster Analysis
MH  - Cytoplasm/microbiology
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Fresh Water
MH  - In Situ Hybridization, Fluorescence
MH  - Microbial Consortia
MH  - Microscopy, Electron, Transmission
MH  - Molecular Sequence Data
MH  - Paramecium/*microbiology
MH  - Phylogeny
MH  - Ponds/*microbiology
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
OTO - NOTNLM
OT  - Bacterial symbiont
OT  - Digestion-resistant bacteria
OT  - Endosymbiont
OT  - FISH
OT  - OD1
OT  - OD1-p
OT  - Protozoa
OT  - Rare biosphere
OT  - SSU rRNA
OT  - Sonnebornia
EDAT- 2013/11/16 06:00
MHDA- 2014/09/18 06:00
CRDT- 2013/11/16 06:00
PHST- 2013/01/19 00:00 [received]
PHST- 2013/08/29 00:00 [revised]
PHST- 2013/08/30 00:00 [accepted]
PHST- 2013/11/16 06:00 [entrez]
PHST- 2013/11/16 06:00 [pubmed]
PHST- 2014/09/18 06:00 [medline]
AID - S0723-2020(13)00157-4 [pii]
AID - 10.1016/j.syapm.2013.08.007 [doi]
PST - ppublish
SO  - Syst Appl Microbiol. 2014 Feb;37(1):35-41. doi: 10.1016/j.syapm.2013.08.007. Epub 
      2013 Nov 11.