PMID- 24233434
OWN - NLM
STAT- MEDLINE
DCOM- 20150223
LR  - 20220408
IS  - 1432-2013 (Electronic)
IS  - 0031-6768 (Print)
IS  - 0031-6768 (Linking)
VI  - 466
IP  - 8
DP  - 2014 Aug
TI  - The distinct roles of anion transporters Slc26a3 (DRA) and Slc26a6 (PAT-1) in 
      fluid and electrolyte absorption in the murine small intestine.
PG  - 1541-56
AB  - The mixing of gastric and pancreatic juice subjects the jejunum to unique ionic 
      conditions with high luminal CO2 tension and HCO3 - concentration. We 
      investigated the role of the small intestinal apical anion exchangers PAT-1 
      (Slc26a6) and DRA (Slc26a3) in basal and CO2/HCO3 --stimulated jejunal fluid 
      absorption. Single pass perfusion of jejunal segments was performed in 
      anaesthetised wild type (WT) as well as in mice deficient in DRA, PAT-1, Na+/H+ 
      exchanger 3 (NHE3) or NHE2, and in carbonic anhydrase II (CAII). Unbuffered 
      saline (pH 7.4) perfusion of WT jejunum resulted in fluid absorption and 
      acidification of the effluent. DRA-deficient jejunum absorbed less fluid than WT, 
      and acidified the effluent more strongly, consistent with its action as a 
      Cl-/HCO3 - exchanger. PAT-1-deficient jejunum also absorbed less fluid but 
      resulted in less effluent acidification. Switching the luminal solution to a 5 % 
      CO2/HCO3 - buffered solution (pH 7.4), resulted in a decrease in jejunal 
      enterocyte pHi in all genotypes, an increase in luminal surface pH and a strong 
      increase in fluid absorption in a PAT-1- and NHE3- but not DRA-, CAII, or 
      NHE2-dependent fashion. Even in the absence of luminal Cl-, luminal CO2/HCO3 - 
      augmented fluid absorption in WT, CAII, NHE2- or DRA-deficient, but not in PAT-1- 
      or NHE3-deficient mice, indicating the likelihood that PAT-1 serves to import 
      HCO3 - and NHE3 serves to import Na+ under these circumstances. The results 
      suggest that PAT-1 plays an important role in jejunal Na+HCO3 - reabsorption, 
      while DRA absorbs Cl- and exports HCO3 - in a partly CAII-dependent fashion. Both 
      PAT-1 and DRA significantly contribute to intestinal fluid absorption and 
      enterocyte acid/base balance but are activated by different ion gradients.
FAU - Xia, Weiliang
AU  - Xia W
FAU - Yu, Qin
AU  - Yu Q
FAU - Riederer, Brigitte
AU  - Riederer B
FAU - Singh, Anurag Kumar
AU  - Singh AK
FAU - Engelhardt, Regina
AU  - Engelhardt R
FAU - Yeruva, Sunil
AU  - Yeruva S
FAU - Song, Penghong
AU  - Song P
FAU - Tian, De-An
AU  - Tian DA
FAU - Soleiman, Manoocher
AU  - Soleiman M
FAU - Seidler, Ursula
AU  - Seidler U
LA  - eng
GR  - R01 DK062809/DK/NIDDK NIH HHS/United States
GR  - R56 DK062809/DK/NIDDK NIH HHS/United States
GR  - DK 62809/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Pflugers Arch
JT  - Pflugers Archiv : European journal of physiology
JID - 0154720
RN  - 0 (Antiporters)
RN  - 0 (Slc26a3 protein, mouse)
RN  - 0 (Slc26a6 protein, mouse)
RN  - 0 (Sulfate Transporters)
SB  - IM
MH  - Animals
MH  - Antiporters/*metabolism
MH  - Hydrogen-Ion Concentration
MH  - Immunohistochemistry
MH  - Intestinal Absorption/*physiology
MH  - Intestinal Mucosa/metabolism
MH  - Intestine, Small/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Microscopy, Confocal
MH  - Polymerase Chain Reaction
MH  - Sulfate Transporters
MH  - Water-Electrolyte Balance/*physiology
PMC - PMC4092241
EDAT- 2013/11/16 06:00
MHDA- 2015/02/24 06:00
PMCR- 2013/11/14
CRDT- 2013/11/16 06:00
PHST- 2013/09/01 00:00 [received]
PHST- 2013/10/10 00:00 [accepted]
PHST- 2013/10/09 00:00 [revised]
PHST- 2013/11/16 06:00 [entrez]
PHST- 2013/11/16 06:00 [pubmed]
PHST- 2015/02/24 06:00 [medline]
PHST- 2013/11/14 00:00 [pmc-release]
AID - 1381 [pii]
AID - 10.1007/s00424-013-1381-2 [doi]
PST - ppublish
SO  - Pflugers Arch. 2014 Aug;466(8):1541-56. doi: 10.1007/s00424-013-1381-2.