PMID- 2423594
OWN - NLM
STAT- MEDLINE
DCOM- 19860707
LR  - 20061115
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 136
IP  - 12
DP  - 1986 Jun 15
TI  - The expression of the sIgD isotype in wild-derived mice.
PG  - 4432-7
AB  - IgD and IgM are concomitantly expressed on the surface of most mouse B 
      lymphocytes and both molecules serve as receptor for antigen. In this 
      communication we report that in contrast to IgM, which is expressed in a constant 
      manner on the surface of spleen B lymphocytes of different laboratory and 
      wild-derived mice, IgD expression is variable among the spleen cells of 
      wild-derived mice. SPE, SEI, and SFM mice belonging to the Mus 3 subgroup show a 
      fluorescence profile characterized by a marked diminution in the population of B 
      lymphocytes expressing the IgD isotype; in addition, these cells have a low sIgD 
      density on their membranes. These findings were confirmed by sodium dodecyl 
      sulfate-polyacrylamide gel electrophoresis analysis of the iodinated membrane 
      proteins. Polyclonal in vitro activation with lipopolysaccharide increases the 
      frequency of surface IgD (sIgD)-bearing spleen cells and sIgD density in the SPE 
      strain but decreases both the frequency and the density of IgD bearing cells in 
      the BALB/c strain. This result suggests that delta gene expression is regulated 
      differently in SPE and BALB/c mice. In addition, genetic analysis of sIgD 
      expression in (BALB/c X SPE)F1 hybrids suggests that the proportion of 
      sIgD-bearing cells and sIgD density are independently regulated.
FAU - Mami, F
AU  - Mami F
FAU - Guenet, J L
AU  - Guenet JL
FAU - Perret, R
AU  - Perret R
FAU - Truffa-Bachi, P
AU  - Truffa-Bachi P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Epitopes)
RN  - 0 (Immunoglobulin Allotypes)
RN  - 0 (Immunoglobulin D)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, Antigen, B-Cell)
RN  - 0 (TNP-ficoll)
RN  - 0 (Trinitrobenzenes)
RN  - 25702-74-3 (Ficoll)
SB  - IM
MH  - Animal Population Groups/*immunology
MH  - Animals
MH  - Animals, Wild/*immunology
MH  - Epitopes/analysis
MH  - Ficoll/analogs & derivatives/immunology
MH  - Immunoglobulin Allotypes/*analysis/genetics
MH  - Immunoglobulin D/*analysis/genetics
MH  - Leukocyte Count
MH  - Lipopolysaccharides/pharmacology
MH  - Lymphocyte Activation
MH  - Lymphocytes/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred CBA
MH  - Receptors, Antigen, B-Cell/*analysis/genetics
MH  - Species Specificity
MH  - Spleen
MH  - Trinitrobenzenes/immunology
EDAT- 1986/06/15 00:00
MHDA- 1986/06/15 00:01
CRDT- 1986/06/15 00:00
PHST- 1986/06/15 00:00 [pubmed]
PHST- 1986/06/15 00:01 [medline]
PHST- 1986/06/15 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1986 Jun 15;136(12):4432-7.