PMID- 2423596
OWN - NLM
STAT- MEDLINE
DCOM- 19860707
LR  - 20181130
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 136
IP  - 12
DP  - 1986 Jun 15
TI  - Cross-linking of immunoglobulin E-receptor complexes induces their interaction 
      with the cytoskeleton of rat basophilic leukemia cells.
PG  - 4565-72
AB  - Bridging of immunoglobulin E (IgE)-receptor complexes on rat basophilic leukemia 
      cells by polyclonal anti-IgE antibodies induces a detergent-resistant association 
      of these complexes with the cellular cytoskeleton. In dose-response curves the 
      extent of the cytoskeletal association appears to follow the extent of bridging, 
      continuing to increase beyond where stimulated degranulation is maximal. This 
      stable association is maintained after the aggregated IgE-receptor complexes have 
      been internalized by the cell. Multivalent antigen and trimeric IgE cause less 
      extensive receptor cross-linking than anti-IgE and stimulate degranulation; they 
      also induce receptor association with the cytoskeleton that is revealed only 
      after stabilization by addition of a chemical cross-linking reagent. The ability 
      of a membrane impermeant chemical cross-linker to stabilize this association 
      suggests that the receptor-cytoskeletal interaction may be mediated by a 
      transmembrane protein that is exposed at the cell surface. Monomeric and dimeric 
      IgE bound to receptors fail to induce a stable interaction with the cytoskeleton 
      even in the presence of chemical cross-linkers and are poor (dimers) or 
      insignificant (monomers) stimulators of cellular degranulation. These findings 
      are consistent with a possible relationship between receptor attachment to the 
      cytoskeleton, receptor immobilization as measured by fluorescence photobleaching 
      recovery, and the triggering of cellular degranulation.
FAU - Robertson, D
AU  - Robertson D
FAU - Holowka, D
AU  - Holowka D
FAU - Baird, B
AU  - Baird B
LA  - eng
GR  - AI18306/AI/NIAID NIH HHS/United States
GR  - AI22449/AI/NIAID NIH HHS/United States
GR  - GM07273/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Dinitrobenzenes)
RN  - 0 (Fluoresceins)
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Succinimides)
RN  - 0 (Thiocyanates)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 9002-93-1 (Octoxynol)
RN  - E647932J7Z (bis(sulfosuccinimidyl)suberate)
RN  - EVY5D0U6SH (dithiobis(succinimidylpropionate))
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Animals
MH  - Antibodies, Anti-Idiotypic
MH  - Basophils/immunology/*metabolism
MH  - *Cross-Linking Reagents
MH  - Cytoskeleton/immunology/*metabolism
MH  - Dinitrobenzenes/immunology
MH  - Fluorescein-5-isothiocyanate
MH  - Fluoresceins
MH  - Histamine Release
MH  - Immunoglobulin E/immunology/*metabolism
MH  - Leukemia, Experimental/immunology/*metabolism
MH  - Octoxynol
MH  - Polyethylene Glycols
MH  - Rats
MH  - Receptors, Fc/*metabolism
MH  - Receptors, IgE
MH  - Receptors, Immunologic/*metabolism
MH  - Solubility
MH  - Succinimides
MH  - Thiocyanates
EDAT- 1986/06/15 00:00
MHDA- 1986/06/15 00:01
CRDT- 1986/06/15 00:00
PHST- 1986/06/15 00:00 [pubmed]
PHST- 1986/06/15 00:01 [medline]
PHST- 1986/06/15 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1986 Jun 15;136(12):4565-72.