PMID- 24237155
OWN - NLM
STAT- MEDLINE
DCOM- 20140408
LR  - 20211021
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Print)
IS  - 0009-9104 (Linking)
VI  - 175
IP  - 3
DP  - 2014 Mar
TI  - Functional evaluation of the role of C-type lectin domain family 16A at the 
      chromosome 16p13 locus.
PG  - 485-97
LID - 10.1111/cei.12240 [doi]
AB  - The type 1 diabetes-associated 16p13 locus contains the CLEC16A gene. Its 
      preferential immune cell expression suggests involvement in autoimmunity. Given 
      its elevated expression in dendritic and B cells - known professional 
      antigen-presenting cells (APCs) - we hypothesize that C-type lectin domain family 
      16 member A (CLEC16A) may be involved in T cell co-stimulation and consequent 
      activation and proliferation. We also sought to identify CLEC16A's subcellular 
      localization. The effect of the CLEC16A knock-down (KD) on B cell co-stimulation 
      and activation of T cells was tested in human lymphoblastoid cell lines (LCLs) by 
      co-culture with CD4(+) T cells. T cell activation and proliferation were 
      determined by flow-cytometric analysis of CD69 and CD25 expression and 
      carboxyfluorescein succinimidyl ester (CFSE) dilution, respectively. CLEC16A 
      subcellular localization in K562 cells was examined by immunofluorescence. We 
      show that the CLEC16A KD did not affect the tested indices of lymphoblastoid cell 
      line (LCL) APC capacity. Additionally, the percentage of activated T cells 
      following LCL co-culture was not affected significantly by the CLEC16A KD. T 
      cells co-cultured with KD or control LCLs also exhibited similar cell division 
      profiles. CLEC16A co-localized with an endoplasmic reticulum (ER) marker, 
      suggesting that it may be an ER protein. In conclusion, CLEC16A may not be 
      involved in T cell co-stimulation. Additional studies on CLEC16A, accounting for 
      its ER localization, are needed to uncover its biological role.
CI  - (c) 2013 British Society for Immunology.
FAU - Zouk, H
AU  - Zouk H
AD  - Endocrine Genetics Laboratory, McGill University Health Center, Montreal 
      Children's Hospital Research Institute, McGill University, QC, Montreal, Canada; 
      Department of Human Genetics, McGill University, QC, Montreal, Canada.
FAU - D'Hennezel, E
AU  - D'Hennezel E
FAU - Du, X
AU  - Du X
FAU - Ounissi-Benkalha, H
AU  - Ounissi-Benkalha H
FAU - Piccirillo, C A
AU  - Piccirillo CA
FAU - Polychronakos, C
AU  - Polychronakos C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (CLEC16A protein, human)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Monosaccharide Transport Proteins)
SB  - IM
MH  - Antigen-Presenting Cells/metabolism
MH  - B-Lymphocytes/metabolism
MH  - *Chromosomes, Human, Pair 16
MH  - Coculture Techniques
MH  - Endoplasmic Reticulum/metabolism
MH  - Gene Knockdown Techniques
MH  - *Genetic Loci
MH  - Humans
MH  - K562 Cells
MH  - Lectins, C-Type/*genetics/metabolism
MH  - Leukocytes, Mononuclear/metabolism
MH  - Lymphocyte Activation/immunology
MH  - Monosaccharide Transport Proteins/*genetics/metabolism
MH  - Protein Transport
MH  - T-Lymphocytes/immunology/metabolism
PMC - PMC3927909
OTO - NOTNLM
OT  - T cell activation and proliferation
OT  - co-culture assay
OT  - flow-cytometry
OT  - subcellular localization
EDAT- 2013/11/19 06:00
MHDA- 2014/04/09 06:00
PMCR- 2015/03/01
CRDT- 2013/11/19 06:00
PHST- 2013/11/12 00:00 [accepted]
PHST- 2013/11/19 06:00 [entrez]
PHST- 2013/11/19 06:00 [pubmed]
PHST- 2014/04/09 06:00 [medline]
PHST- 2015/03/01 00:00 [pmc-release]
AID - 10.1111/cei.12240 [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2014 Mar;175(3):485-97. doi: 10.1111/cei.12240.