PMID- 24237266
OWN - NLM
STAT- MEDLINE
DCOM- 20140715
LR  - 20131217
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 85
IP  - 24
DP  - 2013 Dec 17
TI  - Rationally designed nucleobase and nucleotide coordinated nanoparticles for 
      selective DNA adsorption and detection.
PG  - 12144-51
LID - 10.1021/ac4033627 [doi]
AB  - Nanomaterials for DNA adsorption are useful for sequence-specific DNA detection. 
      Current materials for DNA adsorption employ electrostatic attraction, hydrophobic 
      interaction, or pi-pi stacking, none of which can achieve sequence specificity. 
      Specificity might be improved by involving hydrogen bonding and metal 
      coordination. In this work, a diverse range of nucleobase/nucleotide (adenine, 
      adenosine, adenosine 5'-triphosphate (ATP), adenosine 5'-monophosphate (AMP), and 
      guanosine 5'-triphosphate (GTP)) coordinated materials containing various metal 
      ions (Au(III), Ag(I), Ce(III), Gd(III), and Tb(III)) are prepared. In most cases, 
      nanoparticles are formed. These materials have different surface charges, and 
      positively charged particles only show nonspecific DNA adsorption. Negatively 
      charged materials give different adsorption kinetics for different DNA sequences, 
      where complementary DNA homopolymers are adsorbed faster than other sequences. 
      Therefore, the bases in the coordinated materials can still form base pairs with 
      the DNA. The adsorption strength is mainly controlled by the metal ions, where Au 
      shows the strongest adsorption while lanthanides are weaker. These materials can 
      be used as sensors for DNA detection and can also deliver DNA into cells with no 
      detectable toxicity. By tuning the nanoparticle formulation, enhanced detection 
      can be achieved. This study is an important step toward rational design of 
      materials to achieve specific interactions between biomolecules and synthetic 
      nanoparticle surfaces.
FAU - Wang, Feng
AU  - Wang F
AD  - Department of Chemistry, Waterloo Institute for Nanotechnology, University of 
      Waterloo , Waterloo, Ontario N2L 3G1, Canada.
FAU - Liu, Biwu
AU  - Liu B
FAU - Huang, Po-Jung Jimmy
AU  - Huang PJ
FAU - Liu, Juewen
AU  - Liu J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131122
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Nucleotides)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adsorption
MH  - Base Sequence
MH  - Biosensing Techniques/*methods
MH  - DNA/*analysis/*chemistry/genetics
MH  - Metal Nanoparticles/*chemistry
MH  - Nucleotides/*chemistry
EDAT- 2013/11/19 06:00
MHDA- 2014/07/16 06:00
CRDT- 2013/11/19 06:00
PHST- 2013/11/19 06:00 [entrez]
PHST- 2013/11/19 06:00 [pubmed]
PHST- 2014/07/16 06:00 [medline]
AID - 10.1021/ac4033627 [doi]
PST - ppublish
SO  - Anal Chem. 2013 Dec 17;85(24):12144-51. doi: 10.1021/ac4033627. Epub 2013 Nov 22.