PMID- 24237452
OWN - NLM
STAT- MEDLINE
DCOM- 20140715
LR  - 20131122
IS  - 1525-1594 (Electronic)
IS  - 0160-564X (Linking)
VI  - 37
IP  - 11
DP  - 2013 Nov
TI  - Hypothermic machine perfusion versus cold storage in the rescuing of livers from 
      non-heart-beating donor rats.
PG  - 985-91
LID - 10.1111/aor.12235 [doi]
AB  - The aim of this work was to compare the efficiency of cold storage (CS) and 
      hypothermic machine perfusion (HMP) methods of preserving grafts excised from 
      non-heart-beating donors that had suffered 45 minutes of warm ischemia. We 
      developed a new solution for HMP to use in liver transplantation, based on BES, 
      gluconate, and polyethylene glycol (BGP-HMP solution). After 24 h of HMP or CS, 
      livers were reperfused at 37 degrees C with Krebs-Henseleit solution with added dextran. 
      For both procedures, portal pressure and flow were measured and the intrahepatic 
      resistance (IR) was calculated. The pH oscillations and enzyme activities (LDH, 
      AST, and ALT) were evaluated for the perfusion buffer during normothermic 
      reperfusion. O2 consumption of the liver, glycogen production, and bile flow were 
      also measured during the normothermic reperfusion period. Portal flow and IR 
      showed statistical differences (P < 0.05) between the two groups (n = 5). HMP 
      with BGP-HMP solution resulted in higher values of portal flow and lower IR than 
      CS with HTK solution. Enzyme release after 90 min of reperfusion did not show 
      statistical differences between groups. With regard to bile flow and O2 
      consumption, livers preserved by both processes were able to produce bile, but 
      livers preserved with HMP were able to take up more O2 than livers preserved by 
      CS.
CI  - (c) 2013 Wiley Periodicals, Inc. and International Center for Artificial Organs and 
      Transplantation.
FAU - Carnevale, Matias E
AU  - Carnevale ME
AD  - Binational Center (Argentina-Italy) for Research in Clinical and Applied 
      Cryobiology (CAIC), National University of Rosario, Rosario, Argentina.
FAU - Balaban, Cecilia L
AU  - Balaban CL
FAU - Guibert, Edgardo E
AU  - Guibert EE
FAU - Bottai, Hebe
AU  - Bottai H
FAU - Rodriguez, Joaquin V
AU  - Rodriguez JV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131114
PL  - United States
TA  - Artif Organs
JT  - Artificial organs
JID - 7802778
RN  - 0 (Organ Preservation Solutions)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Bile/metabolism
MH  - Cold Temperature
MH  - Equipment Design
MH  - Liver/enzymology/*physiology/ultrastructure
MH  - Male
MH  - Organ Preservation/*instrumentation
MH  - Organ Preservation Solutions/chemistry/metabolism
MH  - Oxygen/metabolism
MH  - Perfusion/*instrumentation
MH  - Rats
MH  - Rats, Wistar
OTO - NOTNLM
OT  - Cold storage
OT  - Hypothermic machine perfusion
OT  - Liver
OT  - Non-heart-beating donors
EDAT- 2013/11/19 06:00
MHDA- 2014/07/16 06:00
CRDT- 2013/11/19 06:00
PHST- 2013/11/19 06:00 [entrez]
PHST- 2013/11/19 06:00 [pubmed]
PHST- 2014/07/16 06:00 [medline]
AID - 10.1111/aor.12235 [doi]
PST - ppublish
SO  - Artif Organs. 2013 Nov;37(11):985-91. doi: 10.1111/aor.12235. Epub 2013 Nov 14.