PMID- 2424024
OWN - NLM
STAT- MEDLINE
DCOM- 19860718
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 83
IP  - 12
DP  - 1986 Jun
TI  - Differential response to the cytopathic effects of human T-cell lymphotropic 
      virus type III (HTLV-III) superinfection in T4+ (helper) and T8+ (suppressor) 
      T-cell clones transformed by HTLV-I.
PG  - 4297-301
AB  - We isolated six human T-cell lymphotropic virus type I (HTLV-I)-transformed 
      T-cell clones carrying the phenotypic markers of helper and suppressor T cells. 
      Five of the transformed T-cell clones produced infectious HTLV-I, but one (clone 
      55) contained a defective provirus and was therefore not competent for viral 
      replication. To test whether there is interference between HTLV-I and the 
      cytopathic virus HTLV-III in infection and/or their biological effects, we 
      superinfected these T-cell clones with HTLV-III. The recipient cells that we used 
      displayed either the OKT4 or the OKT8 membrane antigens (helper or suppressor 
      phenotype, respectively). The superinfection was successful in all cases, 
      regardless of phenotype of the recipient cells and status of viral production. 
      Both HTLV-III and HTLV-I were expressed by the infected cell lines containing 
      complete HTLV-I proviruses, as demonstrated by electron microscopy and 
      immunofluorescence. However, only HTLV-III in the virus mixture obtained from the 
      culture supernatants was transmitted to the human neoplastic T-cell line H9. The 
      nonproducer clone 55 did not express HTLV-I upon superinfection with HTLV-III. 
      HTLV-III exerted its cytopathic effect on all but one of the superinfected T-cell 
      clones 15-20 days after infection. The exception, clone 67, is also the only cell 
      clone that expresses the phenotypic marker of suppressor T lymphocytes (OKT8); 
      the other clones carry the OKT4 antigen, correlated with helper functions. The 
      virus released from the superinfected clone 67 is cytopathic for fresh peripheral 
      and umbilical-cord blood lymphocytes, suggesting that cellular factors, rather 
      than a genetic change in the virus, may be responsible for the lack of cytopathic 
      effect of HTLV-III on the suppressor T-cell clone 67.
FAU - De Rossi, A
AU  - De Rossi A
FAU - Franchini, G
AU  - Franchini G
FAU - Aldovini, A
AU  - Aldovini A
FAU - Del Mistro, A
AU  - Del Mistro A
FAU - Chieco-Bianchi, L
AU  - Chieco-Bianchi L
FAU - Gallo, R C
AU  - Gallo RC
FAU - Wong-Staal, F
AU  - Wong-Staal F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (Antigens, Surface)
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA, Viral)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Antigens, Differentiation, T-Lymphocyte
MH  - Antigens, Surface/analysis
MH  - Antigens, Viral/analysis
MH  - *Cell Transformation, Viral
MH  - Cells, Cultured
MH  - Cytopathogenic Effect, Viral
MH  - DNA, Viral/analysis
MH  - Deltaretrovirus/*genetics/growth & development/immunology
MH  - Humans
MH  - Microscopy, Electron
MH  - RNA-Directed DNA Polymerase/metabolism
MH  - T-Lymphocytes/*microbiology
MH  - T-Lymphocytes, Helper-Inducer/microbiology
MH  - T-Lymphocytes, Regulatory/microbiology
MH  - Time Factors
MH  - Virus Replication
PMC - PMC323719
EDAT- 1986/06/01 00:00
MHDA- 1986/06/01 00:01
PMCR- 1986/12/01
CRDT- 1986/06/01 00:00
PHST- 1986/06/01 00:00 [pubmed]
PHST- 1986/06/01 00:01 [medline]
PHST- 1986/06/01 00:00 [entrez]
PHST- 1986/12/01 00:00 [pmc-release]
AID - 10.1073/pnas.83.12.4297 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1986 Jun;83(12):4297-301. doi: 10.1073/pnas.83.12.4297.