PMID- 24241224
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131119
LR  - 20211021
IS  - 0032-0935 (Print)
IS  - 0032-0935 (Linking)
VI  - 165
IP  - 4
DP  - 1985 Sep
TI  - Purification and characterization of phosphoribulokinase from wheat leaves.
PG  - 507-12
LID - 10.1007/BF00398096 [doi]
AB  - Homogeneous phosphoribulokinase (PRK; ATP: D-ribulose-5-phosphate 
      1-phosphotransferase, EC 2.7.1.19) was isolated from wheat leaves with a specific 
      activity of 15 mukat mg(-1) protein. The purification included ammonium sulfate 
      cuts, isoelectric precipitation, and hydrophobic and affinity chromatography on 
      pentylagarose and Blue Sepharose CL 6B, respectively. Gel filtration of the 
      purified enzyme yielded a 83000 Da protein. Subunits of about 42000 Da were 
      estimated from sodium dodecyl sulfate-polyacrylamide gels. Wheat leaf PRK was 
      stable for at least four weeks when stored at 4 degrees C. Saturation curves for ribulose 
      5-phosphate (Ru5P) and ATP followed Michaelis-Menten kinetics (K m values: K m 
      Ru5P=50-80 muM; K m ATP=70 muM). The saturation curve for MgCl2 was sigmoidal 
      (half-maximal velocity <0.5 mM). The affinity for Ru5P, ATP and Mg(2+) was not 
      affected by pH changes comparable to pH shifts in the stroma. In contrast to 
      chloroplast fructose-bisphosphatase (Zimmermann et al. 1976, Eur. J. Biochem. 70, 
      361-367) the affinity for ligands remained unchanged in the 
      dithiothreitol-activated and in the non-activated state. The activity of PRK was 
      increasingly sensitive to inhibition by 3-phosphoglyceric acid with decreasing pH 
      below pH 8.0.
FAU - Surek, B
AU  - Surek B
AD  - Botanisches Institut der Westfalischen Wilhelms-Universitat, Schlossgarten 3, 
      D-4400, Munster, Federal Republic of Germany.
FAU - Heilbronn, A
AU  - Heilbronn A
FAU - Austen, A
AU  - Austen A
FAU - Latzko, E
AU  - Latzko E
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Planta
JT  - Planta
JID - 1250576
EDAT- 1985/09/01 00:00
MHDA- 1985/09/01 00:01
CRDT- 2013/11/19 06:00
PHST- 1984/12/01 00:00 [received]
PHST- 1985/02/16 00:00 [accepted]
PHST- 2013/11/19 06:00 [entrez]
PHST- 1985/09/01 00:00 [pubmed]
PHST- 1985/09/01 00:01 [medline]
AID - 10.1007/BF00398096 [doi]
PST - ppublish
SO  - Planta. 1985 Sep;165(4):507-12. doi: 10.1007/BF00398096.