PMID- 24241385
OWN - NLM
STAT- MEDLINE
DCOM- 20141208
LR  - 20211021
IS  - 1436-2236 (Electronic)
IS  - 1436-2228 (Linking)
VI  - 16
IP  - 3
DP  - 2014 Jun
TI  - Detection of QTL in rainbow trout affecting survival when challenged with 
      Flavobacterium psychrophilum.
PG  - 349-60
LID - 10.1007/s10126-013-9553-9 [doi]
AB  - Bacterial cold water disease (BCWD) causes significant economic loss in salmonid 
      aquaculture. We previously detected genetic variation in survival following 
      challenge with Flavobacterium psychrophilum (Fp), the causative agent of BCWD in 
      rainbow trout (Oncorhynchus mykiss). A family-based selection program to improve 
      resistance was initiated in 2005 at the USDA National Center for Cool and Cold 
      Water Aquaculture. Select crosses were made in 2007 and 2009 to evaluate 
      family-based disease survival using Fp injection challenges. From each putative 
      F(2)/BC(1) family generated in 2009, 200-260 fish were challenged in 4-7 replicates 
      per family. Whole genome QTL scans of three F(2)/BC(1) families were conducted with 
      about 270 informative microsatellite loci per family spaced at an average 
      interval size of 6 cM throughout the rainbow trout genome. Markers on chromosomes 
      containing QTL were further evaluated in three additional F(2)/BC(1) families. The 
      additional F(2)/BC(1) families were sire or dam half-sibs (HS) of the initially 
      genome scanned families. Overall, we identified nine major QTL on seven 
      chromosomes that were significant or highly significant with moderate to large 
      effects of at least 13 % of the total phenotypic variance. The largest effect QTL 
      for BCWD resistance explaining up to 40 % of the phenotypic variance was detected 
      on chromosome OMY8 in family 2009070 and in the combined dam HS family 
      2009069-070. The nine major QTL identified in this study are candidates for fine 
      mapping to identify new markers that are tightly linked to disease resistance 
      loci for using in marker assisted selection strategies.
FAU - Vallejo, Roger L
AU  - Vallejo RL
AD  - National Center for Cool and Cold Water Aquaculture, NAA-ARS-USDA, 11861 Leetown 
      Rd., Kearneysville, WV, 25430, USA.
FAU - Palti, Yniv
AU  - Palti Y
FAU - Liu, Sixin
AU  - Liu S
FAU - Evenhuis, Jason P
AU  - Evenhuis JP
FAU - Gao, Guangtu
AU  - Gao G
FAU - Rexroad, Caird E 3rd
AU  - Rexroad CE 3rd
FAU - Wiens, Gregory D
AU  - Wiens GD
LA  - eng
PT  - Journal Article
DEP - 20131116
PL  - United States
TA  - Mar Biotechnol (NY)
JT  - Marine biotechnology (New York, N.Y.)
JID - 100892712
SB  - IM
MH  - Animals
MH  - Aquaculture/methods
MH  - Breeding/methods
MH  - Crosses, Genetic
MH  - Disease Resistance/*genetics
MH  - Fish Diseases/*genetics/*microbiology
MH  - Flavobacteriaceae Infections/genetics/*veterinary
MH  - *Flavobacterium
MH  - Genetic Linkage
MH  - Genotype
MH  - *Oncorhynchus mykiss
MH  - Quantitative Trait Loci/*genetics
EDAT- 2013/11/19 06:00
MHDA- 2014/12/15 06:00
CRDT- 2013/11/19 06:00
PHST- 2013/07/10 00:00 [received]
PHST- 2013/09/22 00:00 [accepted]
PHST- 2013/11/19 06:00 [entrez]
PHST- 2013/11/19 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - 10.1007/s10126-013-9553-9 [doi]
PST - ppublish
SO  - Mar Biotechnol (NY). 2014 Jun;16(3):349-60. doi: 10.1007/s10126-013-9553-9. Epub 
      2013 Nov 16.