PMID- 24245853
OWN - NLM
STAT- MEDLINE
DCOM- 20140723
LR  - 20131211
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 5
IP  - 23
DP  - 2013 Dec 11
TI  - Laccase-polyacrylonitrile nanofibrous membrane: highly immobilized, stable, 
      reusable, and efficacious for 2,4,6-trichlorophenol removal.
PG  - 12554-60
LID - 10.1021/am403849q [doi]
AB  - Increasing attention has been given to nanobiocatalysis for commercial 
      applications. In this study, laccase was immobilized on polyacrylonitrile (PAN) 
      nanofibrous membranes through ethanol/HCl method of amidination reaction and 
      successfully applied for removal of 2,4,6-trichlorophenol (TCP) from water. PAN 
      membranes with fiber diameters from 200 nm to 300 nm were fabricated via 
      electrospinning and provided a large surface area for enzyme immobilization and 
      catalytic reactions. Images of scanning electron microscope demonstrated the 
      enzyme molecules were aggregated on the nanofiber surface. The immobilized 
      laccase exhibited 72% of the free enzyme activity and kept 60% of its initial 
      activity after 10 operation cycles. Moreover, the storage stability of the 
      immobilized laccase was considered excellent because they maintained more than 
      92% of the initial activity after 18 days of storage, whereas the free laccase 
      retained only 20%. The laccase-PAN nanofibrous membranes exhibited high removal 
      efficiency of TCP under the combined actions of biodegradation and adsorption. 
      More than 85% of the TCP was removed under optimum conditions. Effects of various 
      factors on TCP removal efficiency of the immobilized laccase were analyzed. 
      Results suggest that laccase-PAN nanofibrous membranes can be used in removing 
      TCP from aqueous sources and have potential for use in other commercial 
      applications.
FAU - Xu, Ran
AU  - Xu R
AD  - State Key Laboratory of Pollution Control and Resource Reuse, College of 
      Environmental Science and Engineering, Tongji University , Shanghai 200092, P. R. 
      China.
FAU - Chi, Chenglong
AU  - Chi C
FAU - Li, Fengting
AU  - Li F
FAU - Zhang, Bingru
AU  - Zhang B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131122
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 0 (Acrylic Resins)
RN  - 0 (Chlorophenols)
RN  - 0 (Membranes, Artificial)
RN  - 25014-41-9 (polyacrylonitrile)
RN  - EC 1.10.3.2 (Laccase)
RN  - MHS8C5BAUZ (2,4,6-trichlorophenol)
SB  - IM
MH  - Acrylic Resins/*chemistry
MH  - Chlorophenols/*chemistry
MH  - Hydrogen-Ion Concentration
MH  - Laccase/*chemistry
MH  - *Membranes, Artificial
MH  - Microscopy, Electron, Scanning
MH  - *Nanofibers
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Temperature
EDAT- 2013/11/20 06:00
MHDA- 2014/07/24 06:00
CRDT- 2013/11/20 06:00
PHST- 2013/11/20 06:00 [entrez]
PHST- 2013/11/20 06:00 [pubmed]
PHST- 2014/07/24 06:00 [medline]
AID - 10.1021/am403849q [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2013 Dec 11;5(23):12554-60. doi: 10.1021/am403849q. 
      Epub 2013 Nov 22.