PMID- 24246084
OWN - NLM
STAT- MEDLINE
DCOM- 20140915
LR  - 20151119
IS  - 0253-9624 (Print)
IS  - 0253-9624 (Linking)
VI  - 47
IP  - 8
DP  - 2013 Aug
TI  - [Analyzed the molecular interaction network of tumor suppressor gene 14-3-3 sigma 
      in lung cancer cell based on stable isotope labeling by amino acids in cell 
      culture technology].
PG  - 752-6
AB  - OBJECTIVE: To analysis the molecular interaction network of 14-3-3 sigma in non 
      small cell lung cancer (NSCLC) cells. METHODS: Established stable over-expressed 
      14-3-3 sigma protein PG cells, MTT assay was used to assess the growth rate of PG 
      cells. Though stable isotope labeling by amino acids in cell culture (SILAC) and 
      Mass spectrometry (MS) technology, to identify difference expressed proteins 
      caused by over expressed 14-3-3 sigma. The protein expressed >2 or <0.5 times was 
      termed as the differential protein. By searching Human protein reference database 
      (HPRD) and Kyoto encyclopedia of genes and genomes (KEGG), established the 
      molecular interaction network of tumor suppressor gene 14-3-3 sigma. RESULTS: The 
      growth rate of over-expressed 14-3-3 sigma PG cell was obviously slower down 
      compared to vector PG cells. A database including 147 differential protein was 
      established. And a molecular interaction network of 14-3-3 sigma containing 26 
      protein was constructed.In this network, the expression of CSNK2A1 (casein kinase 
      II subunit alpha), involved in numerous cellular processes, such as cell cycle 
      progression, apoptosis and transcription, was the most significantly increased. A 
      DNA repair protein, MEN1 (Menin) which functions as a transcriptional regulator 
      was the most significantly decreased. CONCLUSION: After stable transfected with 
      14-3-3 sigma gene, growth rate of PG cells was inhibited, the proteins associated 
      with cell cycle, DNA damage repair mechanisms were significantly changed, and 
      constructed the molecular interaction network.
FAU - Xiao, Ting
AU  - Xiao T
AD  - State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking 
      Union Medical College & Chinese Academy of Medical Sciences, Beijing 100021, 
      China.
FAU - Mi, Wei
AU  - Mi W
FAU - Li, Min
AU  - Li M
FAU - Cao, Bang-rong
AU  - Cao BR
FAU - Feng, Lin
AU  - Feng L
FAU - Cheng, Shu-jun
AU  - Cheng SJ
FAU - Gao, Yan-ning
AU  - Gao YN
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yu Fang Yi Xue Za Zhi
JT  - Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]
JID - 7904962
RN  - 0 (14-3-3 Proteins)
RN  - 0 (Amino Acids)
RN  - 0 (Biomarkers, Tumor)
RN  - EC 3.1.- (Exoribonucleases)
RN  - EC 3.1.- (SFN protein, human)
SB  - IM
MH  - 14-3-3 Proteins/*genetics
MH  - Amino Acids
MH  - Biomarkers, Tumor/*genetics
MH  - Carcinoma, Non-Small-Cell Lung/genetics
MH  - Cell Cycle
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Exoribonucleases/*genetics
MH  - Humans
MH  - Isotope Labeling/methods
MH  - Lung Neoplasms/*genetics
MH  - Mass Spectrometry
MH  - Transfection
EDAT- 2013/11/20 06:00
MHDA- 2014/09/16 06:00
CRDT- 2013/11/20 06:00
PHST- 2013/11/20 06:00 [entrez]
PHST- 2013/11/20 06:00 [pubmed]
PHST- 2014/09/16 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yu Fang Yi Xue Za Zhi. 2013 Aug;47(8):752-6.