PMID- 2424620
OWN - NLM
STAT- MEDLINE
DCOM- 19860728
LR  - 20190720
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 96
IP  - 1
DP  - 1985 Nov
TI  - Lack of detectable immunoglobulin E receptor expression on 33 of 34 cell lines 
      with natural killer-like or cytotoxic-T-lymphocyte activity.
PG  - 223-30
AB  - We recently reported that a cloned murine cell line with "natural killer 
      (NK)-like" cytolytic function and prominent cytoplasmic granules also expressed 
      large numbers of plasma membrane receptors (Fc epsilon R) which bound mouse 
      immunoglobulin E (IgE) with high affinity (S.J. Galli et al., 1982, Nature 
      (London) 298, 288). We have now performed IgE-binding studies with 31 additional 
      cloned murine cell lines exhibiting "NK-like" lytic activity (defined as the 
      ability to kill YAC-1 lymphoma cells) and three antigen-specific cytotoxic-T-cell 
      clones. One of the NK-like clones expressed a small number of Fc epsilon R (3.0 X 
      10(4)/cell) on one of the two occasions it was tested. None of the other clones, 
      which were derived by several different approaches and which had a variety of 
      surface glycoprotein phenotypes, expressed any detectable specific binding of 
      mouse IgE. By contrast, mast cell clones consistently expressed large numbers of 
      Fc epsilon R. The expression of large numbers of high-affinity Fc epsilon R would 
      appear to represent a very uncommon characteristic of NK-like murine cell lines 
      isolated under conditions similar to those described in this report.
FAU - Galli, S J
AU  - Galli SJ
FAU - Brooks, C G
AU  - Brooks CG
FAU - Dvorak, A M
AU  - Dvorak AM
FAU - Ishizaka, T
AU  - Ishizaka T
LA  - eng
GR  - AI 15384/AI/NIAID NIH HHS/United States
GR  - AI 20292/AI/NIAID NIH HHS/United States
GR  - CA 28834/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cytoplasmic Granules/analysis
MH  - Cytotoxicity Tests, Immunologic
MH  - Immunoglobulin E/*metabolism
MH  - Killer Cells, Natural/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Rats
MH  - Rats, Inbred WF
MH  - Receptors, Fc/*analysis
MH  - Receptors, IgE
MH  - Staining and Labeling
MH  - T-Lymphocytes, Cytotoxic/analysis/*metabolism
EDAT- 1985/11/01 00:00
MHDA- 1985/11/01 00:01
CRDT- 1985/11/01 00:00
PHST- 1985/11/01 00:00 [pubmed]
PHST- 1985/11/01 00:01 [medline]
PHST- 1985/11/01 00:00 [entrez]
AID - 0008-8749(85)90353-3 [pii]
AID - 10.1016/0008-8749(85)90353-3 [doi]
PST - ppublish
SO  - Cell Immunol. 1985 Nov;96(1):223-30. doi: 10.1016/0008-8749(85)90353-3.