PMID- 24246721 OWN - NLM STAT- MEDLINE DCOM- 20140410 LR - 20140520 IS - 1383-5718 (Print) IS - 1383-5718 (Linking) VI - 759 DP - 2014 Jan 1 TI - Assessment of retrospective dose estimation, with fluorescence in situ hybridization (FISH), of six victims previously exposed to accidental ionizing radiation. PG - 1-8 LID - S1383-5718(13)00319-7 [pii] LID - 10.1016/j.mrgentox.2013.07.016 [doi] AB - The present study aims to evaluate the use of the fluorescence in situ hybridization (FISH) translocation assay for retrospective dose estimation of acute accidental exposure to radiation in the past. Reciprocal translocation analysis by FISH with three whole-chromosome probes was performed on normal peripheral blood samples. Samples were irradiated with 0-5Gy (60)Co gamma-rays in vitro, and dose-effect curves were established. FISH-based translocation analyses for six accident victims were then performed, and biological doses were estimated retrospectively by comparison with the dose-effect curves. Reconstructed doses by FISH were compared with estimated doses obtained by analysis of di-centrics performed soon after exposure, or with dose estimates from tooth-enamel electron paramagnetic resonance (EPR) data obtained at the same time as the FISH analysis. Follow-up FISH analyses for an adolescent victim were performed. Results showed that dose-effect curves established in the present study follow a linear-quadratic model, regardless of the background translocation frequency. Estimated doses according to two dose-effect curves for all six victims were similar. FISH dose estimations of three adult victims exposed to accidental radiation less than a decade prior to analysis (3, 6, or 7 years ago) were consistent with those estimated with tooth-enamel EPR measurements or analyses of di-centrics. Estimated doses of two other adult victims exposed to radiation over a decade prior to analysis (16 or 33 years ago) were underestimated and two to three times lower than the values obtained from analysis of di-centrics or tooth-enamel EPR. Follow-up analyses of the adolescent victim showed that doses estimated by FISH analysis decrease rapidly over time. Therefore, the accuracy of dose estimates by FISH is acceptable only when analysis is performed less than 7 years after exposure. Measurements carried out more than a decade after exposure through FISH analysis resulted in underestimation of the biological doses compared with values obtained through analysis of di-centrics and tooth-enamel EPR. CI - Copyright (c) 2013 Elsevier B.V. All rights reserved. FAU - Liu, Qing-Jie AU - Liu QJ AD - China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, PR China. Electronic address: qjliu@nirp.cn. FAU - Lu, Xue AU - Lu X AD - China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, PR China. FAU - Zhao, Xiao-Tao AU - Zhao XT AD - Department of Cardiology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, PR China. FAU - Feng, Jiang-Bin AU - Feng JB AD - China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, PR China. FAU - Lu, Yu-Min AU - Lu YM AD - Henan Institute of Occupational Medicine, Zhengzhou 450052, PR China. FAU - Jiang, En-Hai AU - Jiang EH AD - Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, PR China. FAU - Zhang, Shu-Lan AU - Zhang SL AD - Department of Tumor Chemotherapy and Radiation Sickness, Peking University Third Hospital, Beijing 100191, PR China. FAU - Chen, De-Qing AU - Chen DQ AD - China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, PR China. FAU - Jia, Ting-Zhen AU - Jia TZ AD - Department of Tumor Chemotherapy and Radiation Sickness, Peking University Third Hospital, Beijing 100191, PR China. FAU - Liang, Li AU - Liang L AD - Department of Tumor Chemotherapy and Radiation Sickness, Peking University Third Hospital, Beijing 100191, PR China. Electronic address: liang.dr@163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20131116 PL - Netherlands TA - Mutat Res Genet Toxicol Environ Mutagen JT - Mutation research. Genetic toxicology and environmental mutagenesis JID - 101632149 SB - IM MH - Adolescent MH - Adult MH - Cells, Cultured MH - Dose-Response Relationship, Radiation MH - Electron Spin Resonance Spectroscopy MH - Female MH - Follow-Up Studies MH - Humans MH - In Situ Hybridization, Fluorescence/*methods MH - Male MH - Middle Aged MH - *Radiation Dosage MH - *Radioactive Hazard Release MH - Retrospective Studies OTO - NOTNLM OT - Background translocation frequency OT - Fluorescence in situ hybridization OT - Radiation accident OT - Retrospective dose estimation EDAT- 2013/11/20 06:00 MHDA- 2014/04/11 06:00 CRDT- 2013/11/20 06:00 PHST- 2013/03/09 00:00 [received] PHST- 2013/05/25 00:00 [revised] PHST- 2013/07/05 00:00 [accepted] PHST- 2013/11/20 06:00 [entrez] PHST- 2013/11/20 06:00 [pubmed] PHST- 2014/04/11 06:00 [medline] AID - S1383-5718(13)00319-7 [pii] AID - 10.1016/j.mrgentox.2013.07.016 [doi] PST - ppublish SO - Mutat Res Genet Toxicol Environ Mutagen. 2014 Jan 1;759:1-8. doi: 10.1016/j.mrgentox.2013.07.016. Epub 2013 Nov 16.