PMID- 24248860
OWN - NLM
STAT- MEDLINE
DCOM- 20140909
LR  - 20211021
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 39
IP  - 1
DP  - 2014 Jan
TI  - Electrical stimulation induces calcium-dependent neurite outgrowth and immediate 
      early genes expressions of dorsal root ganglion neurons.
PG  - 129-41
LID - 10.1007/s11064-013-1197-7 [doi]
AB  - It has been reported that electrical stimulation (ES) can promote nerve 
      regeneration. One of the key factors leading to the promotion is calcium, which 
      plays a vital role in the regulation on electrical activity of neurons, but the 
      detailed mechanism is still an open question. In this study, ES was used to 
      stimulate cultured dorsal root ganglion neurons (DRGNs) and we've found that ES 
      could greatly promote neurite outgrowth, and calcium signaling was involved in 
      the ES-induced neurite extension in our research. Detailed pharmacological tests 
      indicated that ES-induced elevation of intracellular free calcium concentration 
      ([Ca(2+)] i ) in DRGNs was realized predominately via calcium influx and calcium 
      mobilization, both of which contributed to enhancing neurite outgrowth promoted 
      by ES. Additionally, a calcium-triggered c-fos and brain-derived neurotrophic 
      factor (BDNF) transcription and/or translation were discovered during the 
      ES-induced neurite outgrowth of cultured DRGNs. To our knowledge, this is the 
      first case of detailed snapshots of studying calcium-dependent neurite outgrowth 
      and BDNF and c-fos expressions in DRGNs induced by ES, which may provide 
      experimental evidence for applying ES to promote regeneration of injured nerves 
      and to enhance synthesis of c-fos and BDNF in neurons, and may also help explore 
      the complex molecular cascades underlying the progressive pathophysiological 
      changes in the ES-induced nerve regeneration in future.
FAU - Yan, Xiaodong
AU  - Yan X
AD  - Department of Orthopaedics, Xijing Hospital, Fourth Military Medical University, 
      127 West Changle Road, Xi'an, 710032, China.
FAU - Liu, Juanfang
AU  - Liu J
FAU - Huang, Jinghui
AU  - Huang J
FAU - Huang, Ming
AU  - Huang M
FAU - He, Fei
AU  - He F
FAU - Ye, Zhengxu
AU  - Ye Z
FAU - Xiao, Wei
AU  - Xiao W
FAU - Hu, Xueyu
AU  - Hu X
FAU - Luo, Zhuojing
AU  - Luo Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131119
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Calcium/*physiology
MH  - Calcium Signaling/physiology
MH  - Cells, Cultured
MH  - Electric Stimulation
MH  - Ganglia, Spinal/*physiology
MH  - Genes, Immediate-Early
MH  - Nerve Regeneration/*physiology
MH  - Neurites/*physiology
MH  - Neurons/physiology
MH  - Proto-Oncogene Proteins c-fos/metabolism
MH  - Rats
EDAT- 2013/11/20 06:00
MHDA- 2014/09/10 06:00
CRDT- 2013/11/20 06:00
PHST- 2013/08/19 00:00 [received]
PHST- 2013/11/07 00:00 [accepted]
PHST- 2013/11/03 00:00 [revised]
PHST- 2013/11/20 06:00 [entrez]
PHST- 2013/11/20 06:00 [pubmed]
PHST- 2014/09/10 06:00 [medline]
AID - 10.1007/s11064-013-1197-7 [doi]
PST - ppublish
SO  - Neurochem Res. 2014 Jan;39(1):129-41. doi: 10.1007/s11064-013-1197-7. Epub 2013 
      Nov 19.