PMID- 24250082
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131119
LR  - 20211021
IS  - 0973-029X (Print)
IS  - 1998-393X (Electronic)
IS  - 0973-029X (Linking)
VI  - 17
IP  - 2
DP  - 2013 May
TI  - Confocal microscopy and exfoliative cytology.
PG  - 217-21
LID - 10.4103/0973-029X.119746 [doi]
AB  - CONTEXT: Early detection of potentially malignant lesions and invasive 
      squamous-cell carcinoma in the oral cavity could be greatly improved through 
      techniques that permit visualization of subtle cellular changes indicative of the 
      neoplastic transformation process. One such technique is confocal microscopy. 
      Combining rapidity with reliability, an innovative idea has been put forward 
      using confocal microscope in exfoliative cytology. AIMS: The main objective of 
      this study was to assess confocal microscopy for cytological diagnosis and the 
      results were compared with that of the standard PAP stain. SETTINGS AND DESIGN: 
      Confocal microscope, acridine orange (AO) stain, PAP (Papanicolaou) stain. The 
      study was designed to assess confocal microscopy for cytological diagnosis. In 
      the process, smears of patients with (clinically diagnosed and/or suspected) oral 
      squamous cell carcinoma as well as those of controls (normal people) were stained 
      with acridine orange and observed under confocal microscope. The results were 
      compared with those of the standard PAP method. MATERIALS AND METHODS: Samples of 
      buccal mucosa smears from normal patients and squamous cell carcinoma patients 
      were made, fixed in 100% alcohol, followed by AO staining. The corresponding set 
      of smears was stained with PAP stain using rapid PAP stain kit. The results 
      obtained were compared with those obtained with AO confocal microscopy. RESULTS: 
      The study had shown nuclear changes (malignant cells) in the smears of squamous 
      cell carcinoma patients as increased intensity of fluorescence of the nucleus, 
      when observed under confocal microscope. Acridine orange confocal microscopy 
      showed good amount of sensitivity and specificity (93%) in identifying malignant 
      cells in exfoliative cytological smears. CONCLUSION: Confocal microscopy was 
      found to have good sensitivity in the identification of cancer (malignant) cells 
      in exfoliative cytology, at par with the PAP method. The rapidity of processing 
      and screening a specimen resulted in saving of time. It added a certain amount of 
      objectivity to the process of arriving at a diagnosis.
FAU - Reddy, Shyam Prasad
AU  - Reddy SP
AD  - Department of Oral Pathology, Staff Quarters, Sarda Estate, Aditya Dental College 
      and Hospital, Beed, Maharashtra, India.
FAU - Ramani, Pratibha
AU  - Ramani P
FAU - Nainani, Purshotam
AU  - Nainani P
LA  - eng
PT  - Journal Article
PL  - India
TA  - J Oral Maxillofac Pathol
JT  - Journal of oral and maxillofacial pathology : JOMFP
JID - 101227995
PMC - PMC3830230
OTO - NOTNLM
OT  - Acridine orange staining
OT  - PAP (Papanicolaou) stain
OT  - confocal microscopy
OT  - exfoliative cytology
OT  - squamous cell carcinoma
COIS- Conflict of Interest: None declared.
EDAT- 2013/11/20 06:00
MHDA- 2013/11/20 06:01
PMCR- 2013/05/01
CRDT- 2013/11/20 06:00
PHST- 2013/11/20 06:00 [entrez]
PHST- 2013/11/20 06:00 [pubmed]
PHST- 2013/11/20 06:01 [medline]
PHST- 2013/05/01 00:00 [pmc-release]
AID - JOMFP-17-217 [pii]
AID - 10.4103/0973-029X.119746 [doi]
PST - ppublish
SO  - J Oral Maxillofac Pathol. 2013 May;17(2):217-21. doi: 10.4103/0973-029X.119746.