PMID- 24250582
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131119
LR  - 20211021
IS  - 1735-0328 (Print)
IS  - 1726-6890 (Electronic)
IS  - 1726-6882 (Linking)
VI  - 12
IP  - 1
DP  - 2013 Winter
TI  - Protective Effects of Vitamin C and NAC on the Toxicity of Rifampin on Hepg2 
      Cells.
PG  - 141-6
AB  - Rifampin, an antibiotic widely used for the treatment of mycobacterial 
      infections, produces hepatic, renal and bone marrow toxicity in human and 
      animals. In this study, the protective effects of vitamin C and n-acetylcysteine 
      (NAC) on the toxicity of rifampin on HepG2 cells were investigated. Human 
      hepatoma cells (HepG2) were cultured in 96-well M of rifampin in the presence of 
      microplate and exposed to 10, 20, 50 and 100 vitamin C (0.1 mg/mL) and NAC (0.2 
      mg/mL). Protective effect of the two drugs against rifampin toxicity was assessed 
      by MTT assay. Results show that both vitamin C and NAC significantly inhibited 
      HepG2 cellular damage due to rifampin, and vitamin C was relatively more potent 
      than NAC. Rifampin is metabolized by the liver and its toxic metabolites are 
      responsible for the drug›s hepatic toxicity. Based on our results, it seems that 
      reactive metabolites are the main agents responsible for rifampin hepatotoxicity. 
      The importance of this finding is that if vitamin C or NAC do not affect the 
      antibacterial activity of rifampin, they could be used as preventive agents in 
      rifampin users.
FAU - Vahdati-Mashhadian, Nasser
AU  - Vahdati-Mashhadian N
AD  - Department of Pharmacodynamy and Toxicology, Faculty of Pharmacy, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
FAU - Jafari, Mahmoud Reza
AU  - Jafari MR
FAU - Sharghi, Nasim
AU  - Sharghi N
FAU - Sanati, Toktam
AU  - Sanati T
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Iran J Pharm Res
JT  - Iranian journal of pharmaceutical research : IJPR
JID - 101208407
PMC - PMC3813199
OTO - NOTNLM
OT  - Cell toxicity
OT  - HepG2
OT  - MTT assay
OT  - NAC
OT  - Vitamin C
EDAT- 2013/11/20 06:00
MHDA- 2013/11/20 06:01
PMCR- 2013/01/01
CRDT- 2013/11/20 06:00
PHST- 2013/11/20 06:00 [entrez]
PHST- 2013/11/20 06:00 [pubmed]
PHST- 2013/11/20 06:01 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - ijpr-12-141 [pii]
PST - ppublish
SO  - Iran J Pharm Res. 2013 Winter;12(1):141-6.