PMID- 24251013
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131119
LR  - 20211021
IS  - 2008-5117 (Print)
IS  - 2008-6830 (Electronic)
IS  - 2008-5117 (Linking)
VI  - 5
IP  - 2
DP  - 2013
TI  - Oxidative Versus Thrombotic Stimulation of Platelets Differentially activates 
      Signalling Pathways.
PG  - 61-5
LID - 10.5681/jcvtr.2013.013 [doi]
AB  - INTRODUCTION: Atherosclerosis is one of the inflammatory underlying disease 
      associated by oxidative stress and thrombotic agents. This study aimed to 
      evaluate the potential role of cupper oxidized low-density lipoprotein (OxLDL) 
      and thrombin for inducing mitogen activated protein kinases (MAPKs) in platelets. 
      METHODS: Phosphorylation of P38MAPK, Jun N-terminal Kinase (JNK), and 
      Extracellular signal-regulated kinases (ERK1/2) and P-selectin expression were 
      determined in lysates of washed human platelets pretreated with low doses of 
      thrombin and cu2+-OxLDL By Enzyme-linked immunosorbent assay (ELISA). 
      Pharmacological inhibition was performed by SB203580, PD980559 and SP6000125 for 
      P38MAPK, ERK1/2 and JNK activity, respectively. The ratio of phosphorylated to 
      total protein was used for normalizing the phospho proteins contents of cells. 
      RESULTS: OxLDL and thrombin significantly and differentially increased P-selectin 
      expression (P<0.05), P38MAPK (P<0.05) and c-JNK (P<0.05) and ERK1/2 (P<0.05) 
      phosphorylation in platelets. SB 203580 and SP6000125 significantly decreased 
      P-selectin expression in both oxidative (P<0.05) and thrombotic (P<0.05) 
      activated platelets. CONCLUSION: Our results indicated that MAPK inhibitors can 
      reduce atherothrombotic events via alterations in P-selectin expression 
      suggesting that these inhibitors may be useful in the inhibition of atheroma 
      development.
FAU - Karimi, Pouran
AU  - Karimi P
AD  - Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
FAU - Rashtchizadeh, Nadereh
AU  - Rashtchizadeh N
LA  - eng
PT  - Journal Article
DEP - 20130627
PL  - Iran
TA  - J Cardiovasc Thorac Res
JT  - Journal of cardiovascular and thoracic research
JID - 101528712
PMC - PMC3825387
OTO - NOTNLM
OT  - Atherosclerosis
OT  - C-Jun N-terminal Kinase Extracellular
OT  - P-selectin
OT  - P38-Mitogen Activated Protein Kinase
OT  - Signal-regulated Kinase
EDAT- 2013/11/20 06:00
MHDA- 2013/11/20 06:01
PMCR- 2013/01/01
CRDT- 2013/11/20 06:00
PHST- 2013/04/17 00:00 [received]
PHST- 2013/06/02 00:00 [accepted]
PHST- 2013/11/20 06:00 [entrez]
PHST- 2013/11/20 06:00 [pubmed]
PHST- 2013/11/20 06:01 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - 10.5681/jcvtr.2013.013 [doi]
PST - ppublish
SO  - J Cardiovasc Thorac Res. 2013;5(2):61-5. doi: 10.5681/jcvtr.2013.013. Epub 2013 
      Jun 27.