PMID- 24251583
OWN - NLM
STAT- MEDLINE
DCOM- 20140918
LR  - 20190318
IS  - 1743-6109 (Electronic)
IS  - 1743-6095 (Linking)
VI  - 11
IP  - 2
DP  - 2014 Feb
TI  - Intracavernous delivery of clonal mesenchymal stem cells restores erectile 
      function in a mouse model of cavernous nerve injury.
PG  - 411-23
LID - 10.1111/jsm.12380 [doi]
AB  - INTRODUCTION: Recently, much attention has focused on stem cell therapy; bone 
      marrow-derived stem cells (BMSCs) are one of the most studied mesenchymal stem 
      cells used in the field of erectile dysfunction (ED). However, a major limitation 
      for the clinical application of stem cell therapy is the heterogeneous nature of 
      the isolated cells, which may cause different treatment outcomes. AIM: We 
      investigated the effectiveness of mouse clonal BMSCs obtained from a single 
      colony by using subfractionation culturing method (SCM) for erectile function in 
      a mouse model of cavernous nerve injury (CNI). METHODS: Twelve-week-old C57BL/6J 
      mice were divided into four groups: sham operation group, bilateral CNI group 
      receiving a single intracavernous (IC) injection of phosphate-buffered saline (20 
      muL) or clonal BMSCs (3 x 10(5) cells/20 muL), and receiving a single 
      intraperitoneal (IP) injection of clonal BMSCs (3 x 10(5) cells/20 muL). MAIN 
      OUTCOME MEASURES: The clonal BMSC line was analyzed for cell-surface epitopes by 
      using fluorescence-activated cell sorting and for differentiation potential. Two 
      weeks after CNI and treatment, erectile function was measured by electrically 
      stimulating the cavernous nerve. The penis was harvested for histologic 
      examinations and Western blot analysis. RESULTS: Clonal BMSCs expressed cell 
      surface markers for mesenchymal stem cells and were capable of differentiating 
      into several lineages, including adipogenic, osteogenic, and chondrogenic cells. 
      Both IC and IP injections of clonal BMSCs significantly restored cavernous 
      endothelial and smooth muscle content, and penile nNOS and neurofilament content 
      in CNI mice. IC injection of clonal BMSCs induced significant recovery of 
      erectile function, which reached 90-100% of the sham control values, whereas IP 
      injection of clonal BMSCs partially restored erectile function. CONCLUSION: We 
      established a homogeneous population of mouse clonal BMSCs using SCM; clonal 
      BMSCs successfully restored erectile function in CNI mice. The homogeneous nature 
      of clonal mesenchymal stem cells may allow their clinical applications.
CI  - (c) 2013 International Society for Sexual Medicine.
FAU - Ryu, Ji-Kan
AU  - Ryu JK
AD  - National Research Center for Sexual Medicine and Department of Urology, Inha 
      University School of Medicine, Incheon, Korea.
FAU - Kim, Da-Ham
AU  - Kim DH
FAU - Song, Kang Moon
AU  - Song KM
FAU - Yi, Tacghee
AU  - Yi T
FAU - Suh, Jun-Kyu
AU  - Suh JK
FAU - Song, Sun U
AU  - Song SU
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131120
PL  - Netherlands
TA  - J Sex Med
JT  - The journal of sexual medicine
JID - 101230693
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type I)
RN  - EC 1.14.13.39 (Nos1 protein, mouse)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Separation
MH  - Disease Models, Animal
MH  - Erectile Dysfunction/*etiology/*surgery
MH  - Injections
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Muscle, Smooth/physiology
MH  - Nitric Oxide Synthase Type I/metabolism
MH  - Penile Erection/*physiology
MH  - Penis/enzymology/*innervation/surgery
MH  - Peripheral Nerve Injuries/*complications
MH  - Regeneration
OTO - NOTNLM
OT  - Cavernous Nerve Injury
OT  - Clonal Mesenchymal Stem Cell
OT  - Erectile Dysfunction
OT  - Mouse Model of Erectile Dysfunction
OT  - Stem Cell Therapy
EDAT- 2013/11/21 06:00
MHDA- 2014/09/19 06:00
CRDT- 2013/11/21 06:00
PHST- 2013/11/21 06:00 [entrez]
PHST- 2013/11/21 06:00 [pubmed]
PHST- 2014/09/19 06:00 [medline]
AID - S1743-6095(15)30658-5 [pii]
AID - 10.1111/jsm.12380 [doi]
PST - ppublish
SO  - J Sex Med. 2014 Feb;11(2):411-23. doi: 10.1111/jsm.12380. Epub 2013 Nov 20.