PMID- 24253860
OWN - NLM
STAT- MEDLINE
DCOM- 20150206
LR  - 20211203
IS  - 1476-5438 (Electronic)
IS  - 1018-4813 (Print)
IS  - 1018-4813 (Linking)
VI  - 22
IP  - 6
DP  - 2014 Jun
TI  - Making the genomic leap in HCT: application of second-generation sequencing to 
      clinical advances in hematopoietic cell transplantation.
PG  - 715-23
LID - 10.1038/ejhg.2013.247 [doi]
AB  - Recent developments in second-generation sequencing (SGS) technologies provide an 
      avenue for achieving rapid and accurate high-throughput analysis of human and 
      microbial genomic diversity. SGS technologies have the potential to transform 
      existing medical management of complex and life-threatening medical conditions by 
      enabling clinicians to develop disease-targeted clinical care plans for each 
      patient. In this review, we outline how innovative SGS-based approaches can 
      improve the care of recipients of allogeneic hematopoietic cell transplantation 
      (HCT), a life-saving procedure that carries a 1-year mortality risk of over 30%. 
      We specifically evaluate foreseeable applications of SGS-based technology in 
      facilitating rapid, phase-sensitive human leukocyte antigen (HLA) typing, 
      assessment of non-HLA genomic compatibility, identifying patients at high risk 
      for adverse drug reactions, and post-HCT monitoring for engraftment, minimal 
      residual disease and infection. We conclude that innovative SGS approaches have 
      the capacity to revolutionize the HCT recipient risk assessment process, support 
      non-invasive clinical monitoring and improve patient outcomes, thereby setting 
      the stage for a new era of genomically informed patient-centered medicine.
FAU - Li, Yun R
AU  - Li YR
AD  - 1] Medical Scientist Training Program, Perelman School of Medicine, University of 
      Pennsylvania, Philadelphia, PA, USA [2] Center for Applied Genomics, Abramson 
      Research Center, The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
FAU - Levine, John E
AU  - Levine JE
AD  - Blood and Marrow Transplant Program, University of Michigan, Ann Arbor, MI, USA.
FAU - Hakonarson, Hakon
AU  - Hakonarson H
AD  - 1] Center for Applied Genomics, Abramson Research Center, The Children's Hospital 
      of Philadelphia, Philadelphia, PA, USA [2] Department of Pediatrics, The 
      Children's Hospital of Philadelphia, Philadelphia, PA, USA [3] Department of 
      Pediatrics, Perelman School of Medicine, University of Pennsylvania, 
      Philadelphia, PA, USA.
FAU - Keating, Brendan J
AU  - Keating BJ
AD  - 1] Center for Applied Genomics, Abramson Research Center, The Children's Hospital 
      of Philadelphia, Philadelphia, PA, USA [2] Department of Pediatrics, The 
      Children's Hospital of Philadelphia, Philadelphia, PA, USA [3] Department of 
      Pediatrics, Perelman School of Medicine, University of Pennsylvania, 
      Philadelphia, PA, USA [4] Division of Transplantation, Department of Surgery, 
      Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
LA  - eng
GR  - U01 HG006830/HG/NHGRI NIH HHS/United States
GR  - 3U01HG006830-S1/HG/NHGRI NIH HHS/United States
GR  - U01HG006830/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20131120
PL  - England
TA  - Eur J Hum Genet
JT  - European journal of human genetics : EJHG
JID - 9302235
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Genomics/*methods/trends
MH  - HLA Antigens/genetics
MH  - Hematopoietic Stem Cell Transplantation/*methods/trends
MH  - High-Throughput Nucleotide Sequencing/*methods/trends
MH  - Histocompatibility Testing/*methods/trends
MH  - Humans
MH  - Translational Research, Biomedical/methods/trends
PMC - PMC4023221
EDAT- 2013/11/21 06:00
MHDA- 2015/02/07 06:00
PMCR- 2015/06/01
CRDT- 2013/11/21 06:00
PHST- 2013/02/21 00:00 [received]
PHST- 2013/09/20 00:00 [revised]
PHST- 2013/09/27 00:00 [accepted]
PHST- 2013/11/21 06:00 [entrez]
PHST- 2013/11/21 06:00 [pubmed]
PHST- 2015/02/07 06:00 [medline]
PHST- 2015/06/01 00:00 [pmc-release]
AID - ejhg2013247 [pii]
AID - 10.1038/ejhg.2013.247 [doi]
PST - ppublish
SO  - Eur J Hum Genet. 2014 Jun;22(6):715-23. doi: 10.1038/ejhg.2013.247. Epub 2013 Nov 
      20.