PMID- 24255678
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 6
IP  - 6
DP  - 2013 Dec
TI  - Biological influence of brain-derived neurotrophic factor (BDNF) on colon cancer 
      cells.
PG  - 1475-1481
AB  - Brain-derived neurotrophic factor (BDNF) has been observed to be elevated in 
      solid tumors including colorectal cancer. The present study aimed to investigate 
      the effect of modulation of BDNF at the transcription level on the cellular 
      function of colorectal cells and to increase our understanding of its biological 
      role in human colon cancer. An investigation of a cohort of human colorectal 
      tissues (tumor n=66; normal n=88) using quantitative PCR and immunohistochemistry 
      demonstrated that BDNF is aberrantly expressed in human colon cancer and a 
      significantly raised level of BDNF is associated with its stage at diagnosis. The 
      expression profile of BDNF in human colon cancer cell lines was evaluated using 
      RT-PCR. A set of anti-BDNF ribozymes were used to transfect colon cancer cells in 
      order to generate BDNF knockdown cells to evaluate the effect on growth and 
      apoptosis. BDNF gene transcripts were successfully detected in the colon cancer 
      cell lines, Caco-2 and HRT18. BDNF knockdown in Caco-2 and HRT18 cell lines 
      resulted in decreased rates of growth and proliferation. Analysis of apoptosis 
      showed that cell apoptosis was increased. It is concluded that BDNF, a 
      neurotrophic growth factor aberrantly expressed in cancers such as colon cancer, 
      has a profound impact on the cellular behavior of colon cancer cells and that 
      BDNF is associated with a reduction in the apoptosis of colon cancer. BDNF is 
      therefore a potential therapeutic target in colon cancer and its effect in human 
      colon cancer requires further investigation.
FAU - Yang, Xiaomei
AU  - Yang X
AD  - Metastasis and Angiogenesis Research Group, Cardiff University School of 
      Medicine, Cardiff CF14 4XN, UK ; Cardiff University-Capital Medical University 
      Joint Centre for Biomedical Research, School of Basic Medical Science, Capital 
      Medical University, Beijing 100069, P.R. China ; Department of Biochemistry and 
      Molecular Biology, Capital Medical University, Beijing 100069, P.R. China.
FAU - Martin, Tracey A
AU  - Martin TA
FAU - Jiang, Wen Guo
AU  - Jiang WG
LA  - eng
PT  - Journal Article
DEP - 20131007
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC3829751
OTO - NOTNLM
OT  - BDNF
OT  - apoptosis
OT  - colon cancer
OT  - proliferation
EDAT- 2013/11/21 06:00
MHDA- 2013/11/21 06:01
PMCR- 2013/10/07
CRDT- 2013/11/21 06:00
PHST- 2013/03/20 00:00 [received]
PHST- 2013/08/23 00:00 [accepted]
PHST- 2013/11/21 06:00 [entrez]
PHST- 2013/11/21 06:00 [pubmed]
PHST- 2013/11/21 06:01 [medline]
PHST- 2013/10/07 00:00 [pmc-release]
AID - etm-06-06-1475 [pii]
AID - 10.3892/etm.2013.1330 [doi]
PST - ppublish
SO  - Exp Ther Med. 2013 Dec;6(6):1475-1481. doi: 10.3892/etm.2013.1330. Epub 2013 Oct 
      7.