PMID- 24257599
OWN - NLM
STAT- MEDLINE
DCOM- 20140416
LR  - 20211021
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 88
IP  - 3
DP  - 2014 Feb
TI  - Evolutionary conservation of primate lymphocryptovirus microRNA targets.
PG  - 1617-35
LID - 10.1128/JVI.02071-13 [doi]
AB  - Epstein-Barr virus (EBV) and rhesus lymphocryptovirus (rLCV) are closely related 
      gammaherpesviruses in the lymphocryptovirus subgroup that express viral microRNAs 
      (miRNAs) during latent infection. In addition to many host mRNAs, EBV miRNAs are 
      known to target latent viral transcripts, specifically those encoding LMP1, 
      BHRF1, and EBNA2. The mRNA targets of rLCV miRNAs have not been investigated. 
      Using luciferase reporter assays, photoactivatable cross-linking and 
      immunoprecipitation (PAR-CLIP), and deep sequencing, we demonstrate that 
      posttranscriptional regulation of LMP1 expression is a conserved function of 
      lymphocryptovirus miRNAs. Furthermore, the mRNAs encoding the rLCV EBNA2 and 
      BHRF1 homologs are regulated by miRNAs in rLCV-infected B cells. Homologous to 
      sites in the EBV LMP1 and BHRF1 3'-untranslated regions (UTRs), we also 
      identified evolutionarily conserved binding sites for the cellular miR-17/20/106 
      family in the LMP1 and BHRF1 3'UTRs of several primate LCVs. Finally, we 
      investigated the functional consequences of LMP1 targeting by individual EBV BART 
      miRNAs and show that select viral miRNAs play a role in the previously observed 
      modulation of NF-kappaB activation.
FAU - Skalsky, Rebecca L
AU  - Skalsky RL
AD  - Department of Molecular Genetics & Microbiology and Center for Virology, Duke 
      University Medical Center, Durham, North Carolina, USA.
FAU - Kang, Dong
AU  - Kang D
FAU - Linnstaedt, Sarah D
AU  - Linnstaedt SD
FAU - Cullen, Bryan R
AU  - Cullen BR
LA  - eng
GR  - R01-AI067968/AI/NIAID NIH HHS/United States
GR  - R01 AI067968/AI/NIAID NIH HHS/United States
GR  - P30-AI064518/AI/NIAID NIH HHS/United States
GR  - T32 CA009111/CA/NCI NIH HHS/United States
GR  - P30 AI064518/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20131120
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Epstein-Barr Virus Infections/*virology
MH  - *Evolution, Molecular
MH  - *Gene Expression Regulation, Viral
MH  - Herpesvirus 4, Human/chemistry/classification/genetics/metabolism
MH  - Humans
MH  - Lymphocryptovirus/chemistry/classification/*genetics/metabolism
MH  - Macaca mulatta
MH  - MicroRNAs/chemistry/*genetics/metabolism
MH  - Molecular Sequence Data
MH  - Primate Diseases/*virology
MH  - Primates
MH  - RNA, Viral/chemistry/*genetics/metabolism
MH  - Sequence Alignment
MH  - Viral Proteins/*genetics/metabolism
PMC - PMC3911612
EDAT- 2013/11/22 06:00
MHDA- 2014/04/17 06:00
PMCR- 2014/08/01
CRDT- 2013/11/22 06:00
PHST- 2013/11/22 06:00 [entrez]
PHST- 2013/11/22 06:00 [pubmed]
PHST- 2014/04/17 06:00 [medline]
PHST- 2014/08/01 00:00 [pmc-release]
AID - JVI.02071-13 [pii]
AID - 02071-13 [pii]
AID - 10.1128/JVI.02071-13 [doi]
PST - ppublish
SO  - J Virol. 2014 Feb;88(3):1617-35. doi: 10.1128/JVI.02071-13. Epub 2013 Nov 20.