PMID- 24258214
OWN - NLM
STAT- MEDLINE
DCOM- 20141015
LR  - 20231213
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Linking)
VI  - 90
IP  - 1
DP  - 2014 Jan
TI  - Modulation of cell cycle progression in the spermatocyte cell line [GC-2spd(ts) 
      Cell-Line] by cigarette smoke condensate (CSC) via arylhydrocarbon 
      receptor-nuclear factor erythroid 2-related factor 2 (Ahr-Nrf2) pathway.
PG  - 9
LID - 10.1095/biolreprod.113.113225 [doi]
AB  - Prior studies in our laboratory have demonstrated that cigarette smoke condensate 
      (CSC) activates arylhydrocarbon receptor (Ahr) leading to upregulation of several 
      antioxidant enzymes in murine spermatocytes. In this study, we show that exposure 
      of the spermatocyte cell line GC-2spd(ts) to CSC induces an increase in Cyp1a1, 
      demonstrating AHR activation, and simultaneous expression and nuclear 
      translocation of nuclear factor erythroid 2-related factor 2 (NRF2), where it is 
      believed to modulate Ahr expression by a feedback mechanism. Pharmacological 
      inhibition by the AHR-antagonist CH223191 and interference by Ahr- and Nrf2-small 
      interfering RNA followed by quantitative real-time PCR implicate the Ahr-Nrf2 
      pathway in the modulation of DNA damage and growth suppression genes such as 
      Gadd45a and P21 and oxidative stress-related genes Cyp1a1, Nrf2, and Ahrr. Flow 
      cytometry accompanied with cell proliferation assay indicate the CSC induces 
      accumulation of spermatocytes at the S-G2/M phase of the cell cycle. Thus, the 
      data obtained suggest that CSC contains several AHR-agonists that are capable of 
      altering the growth pattern of spermatocytes in vitro through the Ahr-Nrf2 
      signaling mechanism.
FAU - Esakky, Prabagaran
AU  - Esakky P
AD  - Research, Department of Veterans Affairs Medical Center, St. Louis, Missouri.
FAU - Hansen, Deborah A
AU  - Hansen DA
FAU - Drury, Andrea M
AU  - Drury AM
FAU - Moley, Kelle H
AU  - Moley KH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20140116
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Ahr protein, mouse)
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Nfe2l2 protein, mouse)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 0 (Smoke)
SB  - IM
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors/*physiology
MH  - Cell Cycle/*drug effects
MH  - Cell Nucleus/drug effects/metabolism
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Male
MH  - Mice
MH  - NF-E2-Related Factor 2/*physiology
MH  - Protein Transport/drug effects
MH  - Receptors, Aryl Hydrocarbon/*physiology
MH  - Signal Transduction/drug effects
MH  - Smoke/*adverse effects
MH  - Spermatocytes/*cytology/*drug effects/physiology
MH  - Nicotiana/*toxicity
OTO - NOTNLM
OT  - Ahr
OT  - CSC
OT  - Nrf2
OT  - cell cycle
OT  - spermatocytes
EDAT- 2013/11/22 06:00
MHDA- 2014/10/16 06:00
CRDT- 2013/11/22 06:00
PHST- 2013/11/22 06:00 [entrez]
PHST- 2013/11/22 06:00 [pubmed]
PHST- 2014/10/16 06:00 [medline]
AID - biolreprod.113.113225 [pii]
AID - 10.1095/biolreprod.113.113225 [doi]
PST - epublish
SO  - Biol Reprod. 2014 Jan 16;90(1):9. doi: 10.1095/biolreprod.113.113225. Print 2014 
      Jan.