PMID- 24258526
OWN - NLM
STAT- MEDLINE
DCOM- 20141124
LR  - 20211021
IS  - 1432-0932 (Electronic)
IS  - 0940-6719 (Print)
IS  - 0940-6719 (Linking)
VI  - 23
IP  - 3
DP  - 2014 Mar
TI  - Using notochordal cells of developmental origin to stimulate nucleus pulposus 
      cells and bone marrow stromal cells for intervertebral disc regeneration.
PG  - 679-88
LID - 10.1007/s00586-013-3107-8 [doi]
AB  - PURPOSE: Bone marrow stromal cells (BMSCs) have been proposed to complement the 
      declining population of nucleus pulposus cells (NPCs) found in a degenerative 
      intervertebral disc. Although able to stop degeneration, they could not produce 
      enough matrix to restore a healthy state. Looking at development, when a large 
      amount of matrix is produced, the disc also contains notochordal cells (NCs), 
      potential progenitors or regulators of NPCs. The aim of the study was, therefore, 
      to combine NCs to a BMSC/NPC mix and evaluate their effects on cell phenotype and 
      matrix production, in long-term culture. METHODS: In a 3D hydrogel, NCs were 
      co-cultured in different ratios with BMSCs and/or NPCs. Matrix production, cell 
      morphology, and gene expression of disc markers were assessed after 4 weeks of 
      culture. RESULTS: At day 28, BMSCs/NPCs highly expressed disc matrix markers 
      (type II collagen and aggrecan) and produced disc matrix up to 30 % of values 
      obtained for the positive control (BMSCs under TGFbeta stimulation). The addition of 
      NCs only slightly up-regulated marker expression (6-12x increase); an 
      up-regulation not reflected at the matrix level. During the 4 weeks of culture, 
      however, the NC phenotype changed drastically (morphology, disc marker 
      expression). CONCLUSION: In contrast to previously reported short-term studies, 
      long-term co-cultures with NCs had no substantial effects on BMSCs and NPCs, most 
      likely due to the loss of the NC native phenotype during culture. It, therefore, 
      appears critical to maintain this specific phenotype for a long-term effect of 
      the NCs.
FAU - Potier, Esther
AU  - Potier E
AD  - Orthopaedic Biomechanics, Department of Biomedical Engineering, Eindhoven 
      University of Technology, GEM-Z 4.103, P.O. Box 513, 5600 MB, Eindhoven, The 
      Netherlands, e.potier@tue.nl.
FAU - Ito, Keita
AU  - Ito K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131121
PL  - Germany
TA  - Eur Spine J
JT  - European spine journal : official publication of the European Spine Society, the 
      European Spinal Deformity Society, and the European Section of the Cervical Spine 
      Research Society
JID - 9301980
RN  - 0 (Aggrecans)
RN  - 0 (Collagen Type II)
SB  - IM
MH  - Aggrecans/metabolism
MH  - Animals
MH  - Cattle
MH  - Coculture Techniques
MH  - Collagen Type II/metabolism
MH  - Gene Expression
MH  - Intervertebral Disc/*metabolism
MH  - Intervertebral Disc Degeneration/*metabolism/pathology
MH  - Mesenchymal Stem Cells/*cytology
MH  - Notochord/*cytology
MH  - Regeneration
MH  - Swine
PMC - PMC3940810
EDAT- 2013/11/22 06:00
MHDA- 2014/12/15 06:00
PMCR- 2015/03/01
CRDT- 2013/11/22 06:00
PHST- 2013/05/01 00:00 [received]
PHST- 2013/11/07 00:00 [accepted]
PHST- 2013/11/07 00:00 [revised]
PHST- 2013/11/22 06:00 [entrez]
PHST- 2013/11/22 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
PHST- 2015/03/01 00:00 [pmc-release]
AID - 3107 [pii]
AID - 10.1007/s00586-013-3107-8 [doi]
PST - ppublish
SO  - Eur Spine J. 2014 Mar;23(3):679-88. doi: 10.1007/s00586-013-3107-8. Epub 2013 Nov 
      21.