PMID- 2425870
OWN - NLM
STAT- MEDLINE
DCOM- 19860917
LR  - 20211203
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 68
IP  - 2
DP  - 1986 Aug
TI  - Determination of platelet antigens and glycoproteins in the human fetus.
PG  - 488-92
AB  - The autosomal recessive transmission of Glanzmann's thrombasthenia (GT) and 
      Bernard-Soulier syndrome (BSS), together with requests of families who already 
      had children with these diseases, prompted us to investigate the feasibility of 
      their antenatal diagnosis. The preliminary step leading to the early detection of 
      GT or BSS was to characterize, in the normal human fetus, the platelet antigens 
      and glycoproteins (GPs) and to define their normal amounts on the membrane 
      surface. Blood samples from 32 fetuses between 18 to 26 weeks of gestation were 
      collected by direct puncture of the umbilical vein using an ultrasound-guided 
      needle. Polyclonal antibodies from human origin directed against PLA1, Leka 
      antigens, and the GPIIb IIIa complex (IgGL), or murine monoclonal antibodies 
      specific for GPIb (AN51, 6D1), GPIIIa (AP-3), or GPIIb IIIa (AP-2) were studied 
      using platelet suspension immunofluorescence tests. The binding of each antibody 
      was quantified using a cytofluorograph (Ortho 50H). PLA1 and Leka antigens were 
      expressed in normal amounts on fetal platelets as early as 16 weeks of 
      intrauterine life. The GPIIb IIIa complex quantified by polyclonal or monoclonal 
      antibodies was in the same range in fetuses (IgGL = 427 +/- 23 AUF, AP-2 = 459.5 
      +/- 8.5; AP-3 = 536 +/- 14) and in adults (IgGL = 420 +/- 30; AP-2 = 498 +/- 11; 
      AP-3 = 515 +/- 13). The platelet binding of antibodies that recognized GPIb was 
      higher in fetuses (AN51 = 491.5 +/- 14; 6D1 = 479 +/- 15) than in adults (AN51 = 
      426.5 +/- 9; 6D1 = 449 +/- 8.7). These results suggest that immunological 
      techniques can be applied as early as 18 weeks of gestation for the antenatal 
      diagnosis of GT and BSS.
FAU - Gruel, Y
AU  - Gruel Y
FAU - Boizard, B
AU  - Boizard B
FAU - Daffos, F
AU  - Daffos F
FAU - Forestier, F
AU  - Forestier F
FAU - Caen, J
AU  - Caen J
FAU - Wautier, J L
AU  - Wautier JL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, Human Platelet)
RN  - 0 (Epitopes)
RN  - 0 (Glycoproteins)
RN  - 0 (ITGB3 protein, human)
RN  - 0 (Integrin beta3)
RN  - 0 (Isoantigens)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - *Antigens, Human Platelet
MH  - Bernard-Soulier Syndrome/diagnosis
MH  - Epitopes
MH  - Female
MH  - Fetal Blood/analysis/*immunology
MH  - Glycoproteins/*analysis
MH  - Humans
MH  - Integrin beta3
MH  - Isoantigens/*analysis
MH  - Platelet Membrane Glycoproteins
MH  - Pregnancy
MH  - Prenatal Diagnosis
MH  - Receptors, Cell Surface/analysis
MH  - Thrombasthenia/diagnosis
EDAT- 1986/08/01 00:00
MHDA- 1986/08/01 00:01
CRDT- 1986/08/01 00:00
PHST- 1986/08/01 00:00 [pubmed]
PHST- 1986/08/01 00:01 [medline]
PHST- 1986/08/01 00:00 [entrez]
AID - S0006-4971(20)69063-2 [pii]
PST - ppublish
SO  - Blood. 1986 Aug;68(2):488-92.