PMID- 24258981
OWN - NLM
STAT- MEDLINE
DCOM- 20140625
LR  - 20131122
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1102
DP  - 2014
TI  - Chromosomal copy number analysis in melanoma diagnostics.
PG  - 199-226
LID - 10.1007/978-1-62703-727-3_12 [doi]
AB  - The majority of melanocytic neoplasms can be correctly diagnosed using routine 
      histopathologic analysis. However, a significant minority of tumors have 
      ambiguous histopathologic attributes that overlap between melanocytic nevi and 
      melanoma. Ancillary tests that assist in distinguishing potentially lethal 
      melanomas from benign melanocytic nevi with atypical histopathologic features are 
      available, but still need refining.Most melanomas have chromosomal copy number 
      aberrations, frequently involving multiple chromosomes. With rare exceptions, 
      such anomalies are not found in melanocytic nevi. This difference formed the 
      basis to develop assays that can help distinguish melanoma from nevi by 
      fluorescence in situ hybridization (FISH) and comparative genomic hybridization 
      (CGH). FISH can detect chromosomal copy number changes of a limited number of 
      loci within individual cells. By contrast, CGH assesses copy number across the 
      entire genome, but typically is performed on bulk cell populations so that copy 
      number changes in individual cells or subpopulations of cells can go undetected. 
      Both FISH and CGH have been used to provide genomic information in 
      histopathologically ambiguous melanocytic tumors that can assist pathologists 
      make correct diagnoses.
FAU - North, Jeffrey P
AU  - North JP
AD  - Department of Dermatology, University of California San Francisco, San Francisco, 
      CA, USA.
FAU - Vemula, Swapna S
AU  - Vemula SS
FAU - Bastian, Boris C
AU  - Bastian BC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Chromosomes, Human/*genetics
MH  - Comparative Genomic Hybridization
MH  - DNA Copy Number Variations/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Melanoma/*diagnosis/*genetics
MH  - Nucleic Acid Denaturation
MH  - Paraffin Embedding
MH  - Pathology, Molecular/*methods
MH  - Tissue Fixation
EDAT- 2013/11/22 06:00
MHDA- 2014/06/26 06:00
CRDT- 2013/11/22 06:00
PHST- 2013/11/22 06:00 [entrez]
PHST- 2013/11/22 06:00 [pubmed]
PHST- 2014/06/26 06:00 [medline]
AID - 10.1007/978-1-62703-727-3_12 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2014;1102:199-226. doi: 10.1007/978-1-62703-727-3_12.