PMID- 24258987
OWN - NLM
STAT- MEDLINE
DCOM- 20140625
LR  - 20151119
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1102
DP  - 2014
TI  - Genotyping of human leukocyte antigen (HLA) ancestral haplotypes as prognostic 
      marker in cancer using PCR analysis.
PG  - 353-66
LID - 10.1007/978-1-62703-727-3_18 [doi]
AB  - The major histocompatibility complex (MHC) comprises a set of genes that are 
      essential to immunity and surveillance against neoplastic transformation. MHC 
      antigens not only regulate antitumor immune responses in experimental animal 
      models but also directly correlate with survival and prognosis of patients with 
      various types of cancers. Effective recognition of tumor cells by effector T 
      cells may be affected by the genotype and the extent of expression of human 
      leukocyte antigen (HLA)-peptide complexes. Therefore, MHC antigens may serve as 
      potential biomarkers for prognosis and allow selection of cancer patients for 
      specific therapy. We describe PCR-based method to determine the HLA genotype in 
      healthy individuals and patients using blood and tumor tissue as DNA source.
FAU - Villabona, Lisa
AU  - Villabona L
AD  - Department of Oncology-Pathology, Karolinska Institute, Karolinska University 
      Hospital, Stockholm, Sweden.
FAU - Andersson, Emilia
AU  - Andersson E
FAU - Marchesi, Maddalena
AU  - Marchesi M
FAU - Masucci, Giuseppe V
AU  - Masucci GV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Alleles
MH  - Biomarkers, Tumor/*metabolism
MH  - DNA, Neoplasm/isolation & purification
MH  - Female
MH  - Genotyping Techniques/*methods
MH  - HLA Antigens/*genetics
MH  - Haplotypes/*genetics
MH  - Humans
MH  - Kaplan-Meier Estimate
MH  - Lymphocytes/metabolism
MH  - Neoplasms/*genetics/*immunology
MH  - Paraffin Embedding
MH  - Polymerase Chain Reaction/*methods
MH  - Prognosis
MH  - Reproducibility of Results
MH  - Tissue Fixation
EDAT- 2013/11/22 06:00
MHDA- 2014/06/26 06:00
CRDT- 2013/11/22 06:00
PHST- 2013/11/22 06:00 [entrez]
PHST- 2013/11/22 06:00 [pubmed]
PHST- 2014/06/26 06:00 [medline]
AID - 10.1007/978-1-62703-727-3_18 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2014;1102:353-66. doi: 10.1007/978-1-62703-727-3_18.