PMID- 24260198
OWN - NLM
STAT- MEDLINE
DCOM- 20140711
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 11
DP  - 2013
TI  - A novel compound from the mushroom Cryptoporus volvatus inhibits porcine 
      reproductive and respiratory syndrome virus (PRRSV) in vitro.
PG  - e79333
LID - 10.1371/journal.pone.0079333 [doi]
LID - e79333
AB  - Porcine reproductive and respiratory syndrome (PRRS), caused by PRRS virus 
      (PRRSV), is a serious contagious disease in the swine industry. At present, there 
      are no effective control strategies against PRRSV. Thus, there is an urgent need 
      for new treatment regimens that have efficacious antiviral activity to compensate 
      for vaccines. The anti-infective effect of Cryptoporus volvatus has previously 
      been demonstrated in Tradational Chinese Medicine. In this report, we expected to 
      identify a new anti-PRRSV agent in the aqueous extract of C. volvatus, by 
      employing a combination of modern chromatographic purification techniques and 
      indirect immunofluorescence assay (IFA). Our results showed that C. volvatus 
      extracts from every separation step differed in their inhibitory potency on 
      PRRSV. One anti-PRRSV component designated as CM-H-L-5 was isolated from 
      water-soluble fraction of C. volvatus. The inhibition induced by CM-H-L-5 
      occurred in a dose-dependent manner. CM-H-L-5 appeared to be a 
      low-molecular-weight polyol fragment with amide groups and carboxylic acid 
      groups. Collectively, our findings imply that CM-H-L-5 from the aqueous extract 
      of C. volvatus has the potential to be used for anti-PRRSV therapy.
FAU - Ma, Zengqiang
AU  - Ma Z
AD  - State Key Laboratory for Agrobiotechnology and Department of Microbiology, China 
      Agricultural University, Beijing, China.
FAU - Zhang, Weiwei
AU  - Zhang W
FAU - Wang, Li
AU  - Wang L
FAU - Zhu, Mengjuan
AU  - Zhu M
FAU - Wang, Hexiang
AU  - Wang H
FAU - Feng, Wen-Hai
AU  - Feng WH
FAU - Ng, Tzi Bun
AU  - Ng TB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131118
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antiviral Agents)
SB  - IM
MH  - Agaricales/*chemistry
MH  - Animals
MH  - Antiviral Agents/chemistry/*pharmacology
MH  - Chromatography, High Pressure Liquid
MH  - Fluorescent Antibody Technique, Indirect
MH  - Magnetic Resonance Spectroscopy
MH  - Porcine respiratory and reproductive syndrome virus/*drug effects
MH  - Real-Time Polymerase Chain Reaction
MH  - Swine
PMC - PMC3832501
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/11/22 06:00
MHDA- 2014/07/12 06:00
PMCR- 2013/11/18
CRDT- 2013/11/22 06:00
PHST- 2013/07/04 00:00 [received]
PHST- 2013/09/29 00:00 [accepted]
PHST- 2013/11/22 06:00 [entrez]
PHST- 2013/11/22 06:00 [pubmed]
PHST- 2014/07/12 06:00 [medline]
PHST- 2013/11/18 00:00 [pmc-release]
AID - PONE-D-13-27649 [pii]
AID - 10.1371/journal.pone.0079333 [doi]
PST - epublish
SO  - PLoS One. 2013 Nov 18;8(11):e79333. doi: 10.1371/journal.pone.0079333. 
      eCollection 2013.