PMID- 24260241
OWN - NLM
STAT- MEDLINE
DCOM- 20140711
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 11
DP  - 2013
TI  - Comprehensive RNA-Seq expression analysis of sensory ganglia with a focus on ion 
      channels and GPCRs in Trigeminal ganglia.
PG  - e79523
LID - 10.1371/journal.pone.0079523 [doi]
LID - e79523
AB  - The specific functions of sensory systems depend on the tissue-specific 
      expression of genes that code for molecular sensor proteins that are necessary 
      for stimulus detection and membrane signaling. Using the Next Generation 
      Sequencing technique (RNA-Seq), we analyzed the complete transcriptome of the 
      trigeminal ganglia (TG) and dorsal root ganglia (DRG) of adult mice. Focusing on 
      genes with an expression level higher than 1 FPKM (fragments per kilobase of 
      transcript per million mapped reads), we detected the expression of 12984 genes 
      in the TG and 13195 in the DRG. To analyze the specific gene expression patterns 
      of the peripheral neuronal tissues, we compared their gene expression profiles 
      with that of the liver, brain, olfactory epithelium, and skeletal muscle. The 
      transcriptome data of the TG and DRG were scanned for virtually all known 
      G-protein-coupled receptors (GPCRs) as well as for ion channels. The expression 
      profile was ranked with regard to the level and specificity for the TG. In total, 
      we detected 106 non-olfactory GPCRs and 33 ion channels that had not been 
      previously described as expressed in the TG. To validate the RNA-Seq data, in 
      situ hybridization experiments were performed for several of the newly detected 
      transcripts. To identify differences in expression profiles between the sensory 
      ganglia, the RNA-Seq data of the TG and DRG were compared. Among the 
      differentially expressed genes (> 1 FPKM), 65 and 117 were expressed at least 
      10-fold higher in the TG and DRG, respectively. Our transcriptome analysis allows 
      a comprehensive overview of all ion channels and G protein-coupled receptors that 
      are expressed in trigeminal ganglia and provides additional approaches for the 
      investigation of trigeminal sensing as well as for the physiological and 
      pathophysiological mechanisms of pain.
FAU - Manteniotis, Stavros
AU  - Manteniotis S
AD  - Department of Cell Physiology, Ruhr-University Bochum, Bochum, Germany.
FAU - Lehmann, Ramona
AU  - Lehmann R
FAU - Flegel, Caroline
AU  - Flegel C
FAU - Vogel, Felix
AU  - Vogel F
FAU - Hofreuter, Adrian
AU  - Hofreuter A
FAU - Schreiner, Benjamin S P
AU  - Schreiner BS
FAU - Altmuller, Janine
AU  - Altmuller J
FAU - Becker, Christian
AU  - Becker C
FAU - Schobel, Nicole
AU  - Schobel N
FAU - Hatt, Hanns
AU  - Hatt H
FAU - Gisselmann, Gunter
AU  - Gisselmann G
LA  - eng
PT  - Journal Article
DEP - 20131108
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Potassium Channels)
RN  - 0 (Receptors, G-Protein-Coupled)
SB  - IM
MH  - Animals
MH  - Ganglia, Sensory/*metabolism
MH  - Ganglia, Spinal/*metabolism
MH  - High-Throughput Nucleotide Sequencing
MH  - In Situ Hybridization
MH  - Male
MH  - Mice
MH  - Potassium Channels/genetics
MH  - Receptors, G-Protein-Coupled
MH  - Trigeminal Ganglion/*metabolism
PMC - PMC3832644
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/11/22 06:00
MHDA- 2014/07/12 06:00
PMCR- 2013/11/08
CRDT- 2013/11/22 06:00
PHST- 2013/08/15 00:00 [received]
PHST- 2013/10/02 00:00 [accepted]
PHST- 2013/11/22 06:00 [entrez]
PHST- 2013/11/22 06:00 [pubmed]
PHST- 2014/07/12 06:00 [medline]
PHST- 2013/11/08 00:00 [pmc-release]
AID - PONE-D-13-33552 [pii]
AID - 10.1371/journal.pone.0079523 [doi]
PST - epublish
SO  - PLoS One. 2013 Nov 8;8(11):e79523. doi: 10.1371/journal.pone.0079523. eCollection 
      2013.