PMID- 24260641
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131122
LR  - 20211021
IS  - 1993-0771 (Print)
IS  - 1993-078X (Electronic)
IS  - 1993-0771 (Linking)
VI  - 5
IP  - 4
DP  - 2011
TI  - Karyotypes, male meiosis and comparative FISH mapping of 18S ribosomal DNA and 
      telomeric (TTAGG) n repeat in eight species of true bugs (Hemiptera, 
      Heteroptera).
PG  - 355-74
LID - 10.3897/CompCytogen.v5i4.2307 [doi]
AB  - Eight species belonging to five true bug families were analyzed using 
      DAPI/CMA3-staining and fluorescence in situ hybridization (FISH) with telomeric 
      (TTAGG)n and 18S rDNA probes. Standard chromosomal complements are reported for 
      the first time for Deraeocoris rutilus (Herrich-Schaffer, 1838) (2n=30+2m+XY) and 
      Deraeocoris ruber(Linnaeus, 1758) (2n=30+2m+XY) from the family Miridae. Using 
      FISH, the location of a 18S rDNA cluster was detected in these species and in 
      five more species: Megaloceroea recticornis (Geoffroy, 1785) (2n=30+XY) from the 
      Miridae; Oxycarenus lavaterae (Fabricius, 1787) (2n=14+2m+XY) from the Lygaeidae 
      s.l.; Pyrrhocoris apterus (Linnaeus, 1758) (2n=22+X) from the Pyrrhocoridae; 
      Eurydema oleracea (Linnaeus, 1758) (2n=12+XY) and Graphosoma lineatum (Linnaeus, 
      1758) (2n=12+XY) from the Pentatomidae. The species were found to differ with 
      respect to location of a 18S rRNA gene cluster which resides on autosomes in 
      Oxycarenus lavaterae and Pyrrhocoris apterus, whereas it locates on sex 
      chromosomes in other five species. The 18S rDNA location provides the  fi rst 
      physical landmark of the genomes of the species studied. The insect consensus 
      telomeric pentanucleotide (TTAGG)n was demonstrated to be absent in all the 
      species studied in this respect, Deraeocoris rutilus, Megaloceroea recticornis, 
      Cimex lectularius Linnaeus, 1758 (Cimicidae), Eurydema oleracea, and Graphosoma 
      lineatum, supporting the hypothesis that this motif was lost in early evolution 
      of the Heteroptera and secondarily replaced with another motif (yet unknown) or 
      the alternative telomerase-independent mechanisms of telomere maintenance. 
      Dot-blot hybridization analysis of the genomic DNA from Cimex lectularius, Nabis 
      sp. and Oxycarenus lavaterae with (TTAGG)n and six other telomeric probes 
      likewise provided a negative result.
FAU - Grozeva, S
AU  - Grozeva S
AD  - Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 
      Blvd Tsar Osvoboditel 1, Sofia 1000, Bulgaria.
FAU - Kuznetsova, V G
AU  - Kuznetsova VG
FAU - Anokhin, B A
AU  - Anokhin BA
LA  - eng
PT  - Journal Article
DEP - 20111109
PL  - Bulgaria
TA  - Comp Cytogenet
JT  - Comparative cytogenetics
JID - 101503807
PMC - PMC3833783
OTO - NOTNLM
OT  - 18S rDNA
OT  - Cimex
OT  - Deraeocoris
OT  - Eurydema
OT  - FISH
OT  - Graphosoma
OT  - Heteroptera
OT  - Karyotypes
OT  - Megaloceroea
OT  - Nabis
OT  - Oxycarenus
OT  - Pyrrhocoris
OT  - dot-blot
OT  - meiosis
OT  - telomeres
EDAT- 2011/01/01 00:00
MHDA- 2011/01/01 00:01
PMCR- 2011/11/09
CRDT- 2013/11/22 06:00
PHST- 2011/11/01 00:00 [received]
PHST- 2011/11/09 00:00 [accepted]
PHST- 2013/11/22 06:00 [entrez]
PHST- 2011/01/01 00:00 [pubmed]
PHST- 2011/01/01 00:01 [medline]
PHST- 2011/11/09 00:00 [pmc-release]
AID - 10.3897/CompCytogen.v5i4.2307 [doi]
PST - epublish
SO  - Comp Cytogenet. 2011 Nov 9;5(4):355-74. doi: 10.3897/CompCytogen.v5i4.2307. 
      eCollection 2011.