PMID- 24260644
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131122
LR  - 20211021
IS  - 1993-0771 (Print)
IS  - 1993-078X (Electronic)
IS  - 1993-0771 (Linking)
VI  - 5
IP  - 5
DP  - 2011
TI  - Cytogenetic characterization of four species of the genus Hypostomus Lacepede, 
      1803 (Siluriformes, Loricariidae) with comments on its chromosomal diversity.
PG  - 397-410
LID - 10.3897/CompCytogen.v5i5.1589 [doi]
AB  - Cytogenetic analyses were performed on fishes of the genus Hypostomus (Hypostomus 
      ancistroides (Ihering, 1911), Hypostomus strigaticeps (Regan, 1908), Hypostomus 
      regani (Ihering, 1905), and Hypostomus paulinus (Ihering, 1905)) from the seven 
      tributaries of the Paranapanema River Basin (Brazil) by means of different 
      staining techniques (C-, Ag-, CMA3- and DAPI-banding) and fluorescence in situ 
      hybridization (FISH) to detect 18S rDNA sites. All species showed different 
      diploid numbers: 2n=68 (10m+26sm+32st-a) in Hypostomus ancistroides, 2n=72 
      (10m+16sm+46st-a) in Hypostomus strigaticeps, 2n=72 (10m+18sm+44st-a) in 
      Hypostomus regani and 2n=76 (6m+16sm+54st-a) in Hypostomus paulinus. Ag-staining 
      and FISH revealed various numbers and locations of NORs in the group. NORs were 
      usually located terminally on the subtelocentric/acrocentric chromosomes: on the 
      long arm in Hypostomus strigaticeps (2 to 4) and Hypostomus paulinus (2); and on 
      the short arm in Hypostomus ancistroides (2 to 8) and Hypostomus regani (2 to 4). 
      Conspicuous differences in heterochromatin distribution and composition were 
      found among the species, terminally located in some st-a chromosomes in 
      Hypostomus ancistroides, Hypostomus strigaticeps, and Hypostomus paulinus, and 
      interstitially dispersed in most st-a chromosomes, in Hypostomus regani. The 
      fluorochrome staining indicated that different classes of GC and/or AT-rich 
      repetitive DNA evolved in this group. Our results indicate that chromosomal 
      rearrangements and heterochromatin base-pair composition were significant events 
      during the course of differentiation of this group. These features emerge as an 
      excellent cytotaxonomic marker, providing a better understanding of the 
      evolutionary mechanisms underlying the chromosomal diversity in Hypostomus 
      species.
FAU - Rubert, Marceleia
AU  - Rubert M
AD  - Departamento de Genetica e Evolucao, Universidade Federal de Sao Carlos, Rodovia 
      Washington Luis, km 235 - SP-310, P.O. Box 676, CEP 13565-905, Sao Carlos, Sao 
      Paulo, Brazil.
FAU - da Rosa, Renata
AU  - da Rosa R
FAU - Jerep, Fernando Camargo
AU  - Jerep FC
FAU - Bertollo, Luiz Antonio Carlos
AU  - Bertollo LA
FAU - Giuliano-Caetano, Lucia
AU  - Giuliano-Caetano L
LA  - eng
PT  - Journal Article
DEP - 20111222
PL  - Bulgaria
TA  - Comp Cytogenet
JT  - Comparative cytogenetics
JID - 101503807
PMC - PMC3833761
OTO - NOTNLM
OT  - NORs
OT  - chromosome banding
OT  - fluorescence in situ hybridization (FISH)
OT  - fluorochromes
OT  - loricariid catfishes
EDAT- 2011/01/01 00:00
MHDA- 2011/01/01 00:01
PMCR- 2011/12/22
CRDT- 2013/11/22 06:00
PHST- 2011/04/21 00:00 [received]
PHST- 2011/08/08 00:00 [accepted]
PHST- 2013/11/22 06:00 [entrez]
PHST- 2011/01/01 00:00 [pubmed]
PHST- 2011/01/01 00:01 [medline]
PHST- 2011/12/22 00:00 [pmc-release]
AID - 10.3897/CompCytogen.v5i5.1589 [doi]
PST - epublish
SO  - Comp Cytogenet. 2011 Dec 22;5(5):397-410. doi: 10.3897/CompCytogen.v5i5.1589. 
      eCollection 2011.