PMID- 24260653
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131122
LR  - 20231110
IS  - 1993-0771 (Print)
IS  - 1993-078X (Electronic)
IS  - 1993-0771 (Linking)
VI  - 6
IP  - 1
DP  - 2012
TI  - Comparative cytogenetic study on two species of the genus Entedon Dalman, 1820 
      (Hymenoptera, Eulophidae) using DNA-binding fluorochromes and molecular and 
      immunofluorescent markers.
PG  - 79-92
LID - 10.3897/CompCytogen.v6i1.2349 [doi]
AB  - Karyotypes of Entedon cionobius Thomson, 1878 and Entedon cioni Thomson, 1878 
      (Hymenoptera: Eulophidae) were studied using DNA-binding ligands with different 
      base specificity (propidium iodide, chromomycin A3, methyl green and DAPI; all 
      these ligands, except for the last one, were used for the first time in parasitic 
      wasps), C-banding, fluorescence in situ hybridization (FISH) with a 45S rDNA 
      probe and 5-methylcytosine immunodetection. Female karyotypes of both species 
      contain five pairs of relatively large metacentric chromosomes and a pair of 
      smaller acrocentric chromosomes (2n = 12). As in many other Hymenoptera, males of 
      both Entedon Dalman, 1820 species have haploid chromosome sets (n = 6). 
      Fluorochrome staining revealed chromosome-specific banding patterns that were 
      similar between the different fluorochromes, except for the CMA3- and PI-positive 
      and DAPI-negative band in the pericentromeric regions of the long arms of both 
      acrocentric chromosomes. The obtained banding patterns were virtually identical 
      in both species and allowed for the identification of each individual chromosome. 
      C-banding revealed a pattern similar to DAPI staining, although centromeric and 
      telomeric regions were stained more intensively using the former technique. FISH 
      detected a single rDNA site in the same position on the acrocentric chromosomes 
      as the bright CMA3-positive band. Immunodetection of 5-methylcytosine that was 
      performed for the first time in the order Hymenoptera revealed 
      5-methylcytosine-rich sites in the telomeric, centromeric and certain 
      interstitial regions of most of the chromosomes.
FAU - Bolsheva, Nadezhda L
AU  - Bolsheva NL
AD  - Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 
      119991, Russia.
FAU - Gokhman, Vladimir E
AU  - Gokhman VE
FAU - Muravenko, Olga V
AU  - Muravenko OV
FAU - Gumovsky, Alex V
AU  - Gumovsky AV
FAU - Zelenin, Alexander V
AU  - Zelenin AV
LA  - eng
PT  - Journal Article
DEP - 20120223
PL  - Bulgaria
TA  - Comp Cytogenet
JT  - Comparative cytogenetics
JID - 101503807
PMC - PMC3833767
OTO - NOTNLM
OT  - DNA methylation
OT  - Entedon
OT  - Eulophidae
OT  - Hymenoptera
OT  - base-specific fluorochromes
OT  - chromosomes
OT  - fluorescence in situ hybridization (FISH)
OT  - karyotypes
EDAT- 2012/01/01 00:00
MHDA- 2012/01/01 00:01
PMCR- 2012/02/23
CRDT- 2013/11/22 06:00
PHST- 2011/12/01 00:00 [received]
PHST- 2012/02/13 00:00 [accepted]
PHST- 2013/11/22 06:00 [entrez]
PHST- 2012/01/01 00:00 [pubmed]
PHST- 2012/01/01 00:01 [medline]
PHST- 2012/02/23 00:00 [pmc-release]
AID - 10.3897/CompCytogen.v6i1.2349 [doi]
PST - epublish
SO  - Comp Cytogenet. 2012 Feb 23;6(1):79-92. doi: 10.3897/CompCytogen.v6i1.2349. 
      eCollection 2012.