PMID- 24260656 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20131122 LR - 20211021 IS - 1993-0771 (Print) IS - 1993-078X (Electronic) IS - 1993-0771 (Linking) VI - 6 IP - 2 DP - 2012 TI - First karyotype description of Hypostomus iheringii (Regan, 1908): a case of heterochromatic polymorphism. PG - 115-25 LID - 10.3897/CompCytogen.v6i2.2595 [doi] AB - In this study, which is the first karyotype analysis of Hypostomus iheringii, nine specimens collected in Corrego da Lapa (tributary of the Passa-Cinco River) showed a diploid number of 80 chromosomes. Silver nitrate staining and fluorescence in situ hybridization (FISH) with an 18S rDNA probe revealed the presence of multiple nucleolus organizer regions (NORs) (chromosome pairs 13, 20, and 34). FISH with a 5S rDNA probe showed that this cistron was only present in chromosome pair 2. When the karyotypes of individual animals were compared, unique heterochromatic polymorphisms were detected on chromosome pairs 1 and 5. Specifically, specimens had heterochromatic blocks (h+h+) on both chromosomes, one chromosome with heterochromatic blocks (h+h-) or chromosomes that lacked heterochromatic blocks (h-h-). Considering that heteromorphic pattern is not correlated with variation in size, the process of heterochromatinization might act on the long arms of these chromosomes. In summary, all chromosomal markers indicate that the karyotype of Hypostomus iheringii is highly differentiated and that the heterochromatinization of chromosomal segments may have contributed to its karyotypic differentiation. FAU - Traldi, Josiane Baccarin AU - Traldi JB AD - Universidade Federal de Sao Carlos, Departamento de Genetica e Evolucao, Rodovia Washington Luis Km 235, Sao Carlos-SP, 13565-905, Brazil. FAU - Vicari, Marcelo Ricardo AU - Vicari MR FAU - Blanco, Daniel Rodrigues AU - Blanco DR FAU - Martinez, Juliana de Fatima AU - Martinez Jde F FAU - Artoni, Roberto Ferreira AU - Artoni RF FAU - Moreira-Filho, Orlando AU - Moreira-Filho O LA - eng PT - Journal Article DEP - 20120316 PL - Bulgaria TA - Comp Cytogenet JT - Comparative cytogenetics JID - 101503807 PMC - PMC3833790 OTO - NOTNLM OT - Hypostomini OT - chromosome variation OT - fish OT - heterochromatinization EDAT- 2012/01/01 00:00 MHDA- 2012/01/01 00:01 PMCR- 2012/03/16 CRDT- 2013/11/22 06:00 PHST- 2011/12/27 00:00 [received] PHST- 2012/02/15 00:00 [accepted] PHST- 2013/11/22 06:00 [entrez] PHST- 2012/01/01 00:00 [pubmed] PHST- 2012/01/01 00:01 [medline] PHST- 2012/03/16 00:00 [pmc-release] AID - 10.3897/CompCytogen.v6i2.2595 [doi] PST - epublish SO - Comp Cytogenet. 2012 Mar 16;6(2):115-25. doi: 10.3897/CompCytogen.v6i2.2595. eCollection 2012.